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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [14]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- sc-409 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-409, RRID:AB_2263416
- Product name
- Anti-GNA12
- Antibody type
- Polyclonal (Antigen purified)
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
Submitted references Neoplastic transformation induced by the gep oncogenes involves the scaffold protein JNK-interacting leucine zipper protein.
Mitogenic Signaling by the gep Oncogene Involves the Upregulation of S-Phase Kinase-Associated Protein 2.
Protease-activated receptor-1 (PAR1) acts via a novel Galpha13-dishevelled axis to stabilize beta-catenin levels.
Modulation of Rho guanine exchange factor Lfc activity by protein kinase A-mediated phosphorylation.
Genetic disruption of p38alpha Tyr323 phosphorylation prevents T-cell receptor-mediated p38alpha activation and impairs interferon-gamma production.
Identification of a novel amino acid response pathway triggering ATF2 phosphorylation in mammals.
Differential effects of opioid agonists on G protein expression in CHO cells expressing cloned human opioid receptors.
MAPK signalling in cardiovascular health and disease: molecular mechanisms and therapeutic targets.
G alpha12 is targeted to the mitochondria and affects mitochondrial morphology and motility.
Role of p38 mitogen-activated protein kinase in cardiac remodelling.
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Transactivation of platelet-derived growth factor receptor alpha by the GTPase-deficient activated mutant of Galpha12.
Neoplastic transformation by the gep oncogene, Gα12, involves signaling by STAT3
Human cytomegalovirus-encoded G protein-coupled receptor US28 mediates smooth muscle cell migration through Galpha12.
Kashef K, Radhakrishnan R, Lee CM, Reddy EP, Dhanasekaran DN
Neoplasia (New York, N.Y.) 2011 Apr;13(4):358-64
Neoplasia (New York, N.Y.) 2011 Apr;13(4):358-64
Mitogenic Signaling by the gep Oncogene Involves the Upregulation of S-Phase Kinase-Associated Protein 2.
Radhakrishnan R, Ha JH, Dhanasekaran DN
Genes & cancer 2010 Oct;1(10):1033-43
Genes & cancer 2010 Oct;1(10):1033-43
Protease-activated receptor-1 (PAR1) acts via a novel Galpha13-dishevelled axis to stabilize beta-catenin levels.
Turm H, Maoz M, Katz V, Yin YJ, Offermanns S, Bar-Shavit R
The Journal of biological chemistry 2010 May 14;285(20):15137-15148
The Journal of biological chemistry 2010 May 14;285(20):15137-15148
Modulation of Rho guanine exchange factor Lfc activity by protein kinase A-mediated phosphorylation.
Meiri D, Greeve MA, Brunet A, Finan D, Wells CD, LaRose J, Rottapel R
Molecular and cellular biology 2009 Nov;29(21):5963-73
Molecular and cellular biology 2009 Nov;29(21):5963-73
Genetic disruption of p38alpha Tyr323 phosphorylation prevents T-cell receptor-mediated p38alpha activation and impairs interferon-gamma production.
Jirmanova L, Sarma DN, Jankovic D, Mittelstadt PR, Ashwell JD
Blood 2009 Mar 5;113(10):2229-37
Blood 2009 Mar 5;113(10):2229-37
Identification of a novel amino acid response pathway triggering ATF2 phosphorylation in mammals.
Chaveroux C, Jousse C, Cherasse Y, Maurin AC, Parry L, Carraro V, Derijard B, Bruhat A, Fafournoux P
Molecular and cellular biology 2009 Dec;29(24):6515-26
Molecular and cellular biology 2009 Dec;29(24):6515-26
Differential effects of opioid agonists on G protein expression in CHO cells expressing cloned human opioid receptors.
Xu H, Wang X, Partilla JS, Bishop-Mathis K, Benaderet TS, Dersch CM, Simpson DS, Prisinzano TE, Rothman RB
Brain research bulletin 2008 Sep 5;77(1):49-54
Brain research bulletin 2008 Sep 5;77(1):49-54
MAPK signalling in cardiovascular health and disease: molecular mechanisms and therapeutic targets.
Muslin AJ
Clinical science (London, England : 1979) 2008 Oct;115(7):203-18
Clinical science (London, England : 1979) 2008 Oct;115(7):203-18
G alpha12 is targeted to the mitochondria and affects mitochondrial morphology and motility.
Andreeva AV, Kutuzov MA, Voyno-Yasenetskaya TA
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Aug;22(8):2821-31
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2008 Aug;22(8):2821-31
Role of p38 mitogen-activated protein kinase in cardiac remodelling.
Frantz S, Behr T, Hu K, Fraccarollo D, Strotmann J, Goldberg E, Ertl G, Angermann CE, Bauersachs J
British journal of pharmacology 2007 Jan;150(2):130-5
British journal of pharmacology 2007 Jan;150(2):130-5
A single lentiviral vector platform for microRNA-based conditional RNA interference and coordinated transgene expression.
Shin KJ, Wall EA, Zavzavadjian JR, Santat LA, Liu J, Hwang JI, Rebres R, Roach T, Seaman W, Simon MI, Fraser ID
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 12;103(37):13759-64
Proceedings of the National Academy of Sciences of the United States of America 2006 Sep 12;103(37):13759-64
Transactivation of platelet-derived growth factor receptor alpha by the GTPase-deficient activated mutant of Galpha12.
Kumar RN, Ha JH, Radhakrishnan R, Dhanasekaran DN
Molecular and cellular biology 2006 Jan;26(1):50-62
Molecular and cellular biology 2006 Jan;26(1):50-62
Neoplastic transformation by the gep oncogene, Gα12, involves signaling by STAT3
R N Kumar, S K Shore, N Dhanasekaran
Oncogene 2005 Oct;25(6):899-906
Oncogene 2005 Oct;25(6):899-906
Human cytomegalovirus-encoded G protein-coupled receptor US28 mediates smooth muscle cell migration through Galpha12.
Melnychuk RM, Streblow DN, Smith PP, Hirsch AJ, Pancheva D, Nelson JA
Journal of virology 2004 Aug;78(15):8382-91
Journal of virology 2004 Aug;78(15):8382-91
No comments: Submit comment
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- Band of predicted size in kDa (+/-20%) with additional bands present.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 213, 118, 83, 50, 33, 26, 18
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.
