PA5-96649

antibody from Invitrogen Antibodies

Targeting: BECN1 ATG6, VPS30

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96649

Provider

Invitrogen Antibodies

Product name

Beclin 1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: LKLDTSFKIL DRVTIQELTA PLLTTAQAKP GETQEEETNS GEEPFIETPR QDGVSRRFIP PARMMSTESA NSFTLIGEAS DGGTMENLSR RLKVTGDLFD IMSGQTDVDH PLCEECTDTL LDQLDTQLNV TENECQNYKR CLEILEQMNE DDSEQLQMEL KELALEEERL IQELEDVEKN RKIVAENLEK VQAEAERLDQ EEAQYQREYS EFKRQQLELD DELKSVENQM RYAQTQLDKL KKTNVFNATF HIWHSG

Concentration

3.08 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using BECN1 Polyclonal antibody (Product # PA5-96649) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts from normal (control) and BECN1 knockout (KO) 293T cells, using BECN1 Polyclonal antibody (Product # PA5-96649) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 5s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Beclin 1 in extracts of various cell lines using Beclin 1 Polyclonal Antibody (Product # PA5-96649) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Beclin 1 was performed in HeLa cells using Beclin 1 Polyclonal Antibody (Product # PA5-96649).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Beclin 1 in paraffin-embedded human stomach using Beclin 1 Polyclonal Antibody (Product # PA5-96649) at a dilution of 1:200.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Beclin 1 in paraffin-embedded mouse kidney using Beclin 1 Polyclonal Antibody (Product # PA5-96649) at a dilution of 1:200.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunoprecipitation analysis of Beclin 1 was performed in 200 µg extracts of HeLa cells using Beclin 1 Polyclonal Antibody (Product # PA5-96649). Western blot was performed from the immunoprecipitate using Beclin 1 Polyclonal Antibody.