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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
- Immunohistochemistry [1]
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- Product number
- PAB7450 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB7450, RRID:AB_1578182
- Product name
- NRAS polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of NRAS.
- Antigen sequence
C-RMKKLNSSDDGTQ- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Number of nevi and early-life ambient UV exposure are associated with BRAF-mutant melanoma.
Thomas NE, Edmiston SN, Alexander A, Millikan RC, Groben PA, Hao H, Tolbert D, Berwick M, Busam K, Begg CB, Mattingly D, Ollila DW, Tse CK, Hummer A, Lee-Taylor J, Conway K
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2007 May;16(5):991-7
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology 2007 May;16(5):991-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NRAS polyclonal antibody (Cat # PAB7450) (0.01 ug/mL) staining of A-431 lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- 293 overexpressing NRAS and probed with NRAS polyclonal antibody (Cat # PAB7450) (mock transfection in first lane), tested by Origene.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NRAS polyclonal antibody (Cat # PAB7450) (0.1 ug/ml) staining of rat intestines lysate (35 ug protein in RIPA buffer).Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- NRAS polyclonal antibody (Cat # PAB7450) (2.5 ug/mL) staining of paraffin embedded human small intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
