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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- AB0012-200 - Provider product page
- Provider
- SICGEN
- Proper citation
- SICGEN Cat#AB0012-200, RRID:AB_2333168
- Product name
- Anti-Rab5b
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody to Rab5c. Rab5c belongs to the small GTPase superfamily, Rab family. Rab5c is an Early Endosome Marker and functions as a key regulator of vesicular trafficking during early endocytosis.
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Donkey, Feline, Goat, Guinea Pig, Hamster, Horse, Porcine, Rabbit, Sheep, Simian, Other
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 800 µg
- Concentration
- 4 mg/ml
- Storage
- Store at -20 C for long-term storage. Store at 2-8 C for up to one month.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cell lysates at 50 µg per lane
- Sample type
- HEK-293 transfected with GFP-Rab5a, GFP-Rab5b, GFP-Rab5c, MDCK
- Primary Ab dilution
- 1/500
- Conjugate
- Unconjugated
- Secondary Ab
- Secondary Ab
- Secondary Ab dilution
- 1/10,000
Supportive validation
- Submitted by
- SICGEN (provider)
- Main image
- Experimental details
- Cells were fixed with 4% PFA
- Sample type
- B6-RPE07
- Primary Ab dilution
- 1/100
- Conjugate
- Unconjugated
