GTX15750

antibody from GeneTex

Targeting: IFNG

Western blot (Data presented on provider website)

ELISA (Supportive data in Antibodypedia)

Immunohistochemistry (Data presented on provider website)

Flow cytometry (Data presented on provider website)

Antibody Data

Product number

GTX15750

Provider

GeneTex

Product name

Interferon gamma antibody [B133.5]

Antibody type

Monoclonal

Reactivity

Human

Host

Mouse

ELISA

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Purified human CD4+ T cells were left non-stimulated (Lane 1), stimulated with anti-CD3 (Lane 2), or stimulated with anti-CD3 and anti-CD28 (Lane 3) for three days. IFN gamma production (pg/ml) was determined by ELISA by coating IFN gamma antibody [2G1] (1:1000), blocking with 1% BSA in PBS, and detection with IFN gamma antibody [B133.5] (1:1000), followed by peroxidase-conjugated avidin and TMB substrate.

Validation comment

ELISA