Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [6]
- Flow cytometry [2]
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Validation data
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- Product number
- NB100-781 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-781, RRID:AB_10002528
- Product name
- Goat Polyclonal SOCS-1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references A family of cytokine-inducible inhibitors of signalling.
Starr R, Willson TA, Viney EM, Murray LJ, Rayner JR, Jenkins BJ, Gonda TJ, Alexander WS, Metcalf D, Nicola NA, Hilton DJ
Nature 1997 Jun 26;387(6636):917-21
Nature 1997 Jun 26;387(6636):917-21
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SOCS-1 Antibody [NB100-781] - (0.3ug/ml) staining of Human Spelen (A), Pig Pancreas (B), (1ug/ml) Mouse Spleen (C) lysate and negative control HeLa (D) cell lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - (5ug/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Staining of paraffin embedded Human Kidney. Antibody at 8 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Staining of paraffin embedded Mouse Kidney. Antibody at 10 ug/mL. Heat induced antigen retrieval with citrate buffer pH 6, HRP-Staining (1:500).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Negative Control showing staining of paraffin embedded Human Kidney, with no primary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - Negative Control showing staining of paraffin embedded Mouse Kidney, with no primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SOCS-1 Antibody [NB100-781] - analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: SOCS-1 Antibody [NB100-781] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/mL) followed by Alexa Fluor 488 secondary antibody (1 ug/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.