PA5-93046

antibody from Invitrogen Antibodies

Targeting: CCNC CycC

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-93046

Provider

Invitrogen Antibodies

Product name

Cyclin C Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAGNFWQSSH YLQWILDKQD LLKERQKDLK FLSEEEYWKL QIFFTNVIQA LGEHLKLRQQ VIATATVYFK RFYARYSLKS IDPVLMAPTC VFLASKVEEF GVVSNTRLIA AATSVLKTRF SYAFPKEFPY RMNHILECEF YLLELMDCCL IVYHPYRPLL QYVQDMGQED MLLPLAWRIV NDTYRTDLCL LYPPFMIALA CLHVACVVQQ KDARQWFAEL SVDMEKILEI IRVILKLYEQ WKNFDERKEM ATILSKMPKP KPPPNSEGEQ GPNGSQNSSY SQS; Positive Samples: MCF7, NIH/3T3, Mouse liver, Mouse kidney, Rat liver; Cellular Location: Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.95 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of extracts of various cell lines, using CCNC Polyclonal antibody (Product # PA5-93046) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Exposure time: 60s.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of Cyclin C in extracts of various cell lines using Cyclin C Polyclonal Antibody (Product # PA5-93046) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of Cyclin C was performed in MCF7 cells using Cyclin C Polyclonal Antibody (Product # PA5-93046).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cyclin C in paraffin-embedded human lung cancer using Cyclin C Polyclonal Antibody (Product # PA5-93046) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cyclin C in paraffin-embedded human lung cancer using Cyclin C Polyclonal Antibody (Product # PA5-93046) at a dilution of 1:100.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of Cyclin C in paraffin-embedded human colon carcinoma using Cyclin C Polyclonal Antibody (Product # PA5-93046) at a dilution of 1:200.