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LearnMA5-34793
antibody from Invitrogen Antibodies
Targeting: CHAF1A
CAF-1, CAF1, CAF1B, CAF1P150, MGC71229, P150
Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- MA5-34793 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CAF1 p150 Recombinant Rabbit Monoclonal Antibody (JG38-98)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: K562, A431, 293T, LOVO, human tonsil tissue, human colon tissue, Daudi.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG38-98
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of p150 CAF1 in K562 (1) and A431 (2) cell lysate. Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), at a dilution of 1:1000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p150 CAF1 in LOVO cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p150 CAF1 in A431 cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of p150 CAF1 in 293T cells (green). Samples were fixed in paraformaldehyde and permeabilised with 0.25% Triton X100/PBS, incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), followed by DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of p150 CAF1 in paraffin-embedded human tonsil tissue. Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), and followed by hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of p150 CAF1 in paraffin-embedded human colon tissue. Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793), and followed by hematoxylin.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of p150 CAF1 in Daudi cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with p150 CAF1 monoclonal antibody (Product # MA5-34793) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
