ALX-803-321-C100

antibody from Enzo Life Sciences

Targeting: VRK1

Western blot (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

ALX-803-321-C100

Provider

Enzo Life Sciences

Proper citation

Enzo Life Sciences Cat#ALX-803-321-C100, RRID:AB_2052828

Product name

VRK1 (human) (1F6) monoclonal antibody

Antibody type

Monoclonal

Antigen

Recombinant full length protein

Description

Protein G-affinity purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1F6

Vial size

100 μg

Storage

-20°C

Handling

Avoid freeze/thaw cycles. After opening, prepare aliquots and store at -20¡C.

References

Submitted references

Plk3 interacts with and specifically phosphorylates VRK1 in Ser342, a downstream target in a pathway that induces Golgi fragmentation.

López-Sánchez I, Sanz-García M, Lazo PA
Molecular and cellular biology 2009 Mar;29(5):1189-201

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A central role for CK1 in catalyzing phosphorylation of the p53 transactivation domain at serine 20 after HHV-6B viral infection.

MacLaine NJ, Oster B, Bundgaard B, Fraser JA, Buckner C, Lazo PA, Meek DW, Höllsberg P, Hupp TR
The Journal of biological chemistry 2008 Oct 17;283(42):28563-73

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Proteomics identification of nuclear Ran GTPase as an inhibitor of human VRK1 and VRK2 (vaccinia-related kinase) activities.

Sanz-García M, López-Sánchez I, Lazo PA
Molecular & cellular proteomics : MCP 2008 Nov;7(11):2199-214

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Human VRK1 is an early response gene and its loss causes a block in cell cycle progression.

Valbuena A, López-Sánchez I, Lazo PA
PloS one 2008 Feb 20;3(2):e1642

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Identification of a dominant epitope in human vaccinia-related kinase 1 (VRK1) and detection of different intracellular subpopulations.

Valbuena A, López-Sánchez I, Vega FM, Sevilla A, Sanz-García M, Blanco S, Lazo PA
Archives of biochemistry and biophysics 2007 Sep 1;465(1):219-26

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VRK1 signaling pathway in the context of the proliferation phenotype in head and neck squamous cell carcinoma.

Santos CR, Rodríguez-Pinilla M, Vega FM, Rodríguez-Peralto JL, Blanco S, Sevilla A, Valbuena A, Hernández T, van Wijnen AJ, Li F, de Alava E, Sánchez-Céspedes M, Lazo PA
Molecular cancer research : MCR 2006 Mar;4(3):177-85

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p53 Stabilization and accumulation induced by human vaccinia-related kinase 1.

Vega FM, Sevilla A, Lazo PA
Molecular and cellular biology 2004 Dec;24(23):10366-80

Immunocytochemistry

Supportive validation

Submitted by

Enzo Life Sciences (provider)

Main image

Experimental details

ÊWestern BlotÊof endogenous Vaccinia-related Kinase 1 (VRK1) (human) by the MAb to VRK1(1F6) (Prod. No. ALX-803-321). Method: Total cell extracts (10µg) of human embryo kidney 293 (HEK293) (lane 1) and HeLa (lane 2) cell lines were run on a SDS-gel, and revealed with MAb to VRK1(1F6) (1:1'000) for 1 hour at room temperature followed by incubation with HRP-coupled goat-anti mouse antibody (1:4'000). The arrow indicates endogenous VRK1.Ê