LS-C114665

antibody from LSBio

Targeting: LDHA

Western blot (Data presented on provider website)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

LS-C114665

Provider

LSBio

Product name

LDHA / LDH1 Antibody (clone OTI2D11) LS-C114665

Antibody type

Monoclonal

Description

Protein A/G purified

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

OTI2D11

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Immunoprecipitation

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Immunoprecipitation(IP) of LDHA by using monoclonal anti-LDHA antibodies (Negative control: IP without adding anti-LDHA antibody.). For each experiment, 500ul of DDK tagged LDHA overexpression lysates (at 1:5 dilution with HEK293T lysate), 2 ug of anti-LDHA antibody and 20ul (0.1 mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Immunohistochemistry

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Enhanced method

Genetic validation

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of breast tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of thyroid tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of ovary tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of pancreas tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Adenocarcinoma of endometrium tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

IHC of paraffin-embedded Carcinoma of bladder tissue using anti-LDHA mouse monoclonal antibody. (Dilution 1:50).