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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- MAB9158-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Rat Lactate Dehydrogenase A/LDHA Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human LDHA in direct ELISAs and Western blots.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antigen sequence
P00338- Isotype
- IgG
- Antibody clone number
- 2066C
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human and Rat Lactate Dehydrogenase A/LDHA by Western Blot. Western blot shows lysates of Daudi human Burkitt's lymphoma cell line, HepG2 human hepatocellular carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and rat skeletal muscle tissue. PVDF membrane was probed with 0.2 µg/mL of Rabbit Anti-Human/Rat Lactate Dehydrogenase A/LDHA Monoclonal Antibody (Catalog # MAB9158) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Lactate Dehydrogenase A/LDHA at approximately 34-36 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lactate Dehydrogenase A/LDHA in PC-3 Human Cell Line. Lactate Dehydrogenase A/LDHA was detected in immersion fixed PC-3 human prostate cancer cell line using Rabbit Anti-Human/Rat Lactate Dehydrogenase A/LDHA Monoclonal Antibody (Catalog # MAB9158) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Lactate Dehydrogenase A/LDHA in Human Pancreas. Lactate Dehydrogenase A/LDHA was detected in immersion fixed paraffin-embedded sections of human pancreas using Rabbit Anti-Human/Rat Lactate Dehydrogenase A/LDHA Monoclonal Antibody (Catalog # MAB9158) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
