RQ6383

antibody from NSJ Bioreagents

Targeting: IDO2 INDOL1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

RQ6383

Provider

NSJ Bioreagents

Product name

Indoleamine 2,3-dioxygenase 2 Antibody / IDO2 / INDOL1

Antibody type

Polyclonal

Description

Purified

Reactivity

Human, Mouse, Rat, Simian

Host

Rabbit

Vial size

100 µg

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Storage

After reconstitution, the Indoleamine 2,3-dioxygenase 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of 1) rat liver, 2) mouse liver and 3) monkey kidney tissue lysate with Indoleamine 2,3-dioxygenase 2 antibody. Predicted molecular weight ~47 kDa.

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC staining of FFPE human liver cancer with Indoleamine 2,3-dioxygenase 2 antibody. HIER: boil tissue sections in pH8 EDTA for 20 min and allow to cool before testing.

Flow cytometry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Flow cytometry testing of human K562 cells with Indoleamine 2,3-dioxygenase 2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= Indoleamine 2,3-dioxygenase 2 antibody.