MA1-46422

antibody from Invitrogen Antibodies

Targeting: LMO2 RBTN2, RBTNL1, RHOM2, TTG2

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-46422

Provider

Invitrogen Antibodies

Product name

LMO2 Monoclonal Antibody (1A9-3B11)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Suggested positive control: OCIL-Y19, Raji or Ramos whole cell lysates.

Antibody clone number

1A9-3B11

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot detection of LMO2 in Ramos cell lysate

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of LMO2 in Ramos cell lysate. Sample was incubated in LMO2 monoclonal antibody (Product # MA1-46422).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of LMO2 in tonsil tissue. Samples were incubated in LMO2 monoclonal antibody (Product # MA1-46422). LMO2 (green).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of LMO2 in formalin fixed paraffin-embedded (FFPE) human tonsil. Samples were incubated in LMO2 monoclonal antibody (Product # MA1-46422) using a dilution of 1:500. Bond Rx autostainer (Leica Biosystems). The assay involved 20 minutes of heat induced antigen retrieval (HIER) using 10mM sodium citrate buffer (pH 6.0) and endogenous peroxidase quenching with peroxide block. The sections were incubated with primary antibody for 30 minutes and Bond Polymer Refine Detection (Leica Biosystems) with DAB was used for signal development followed by counterstaining with hematoxylin. Whole slide scanning and capturing of representative images was performed using Aperio AT2 (Leica Biosystems). Nuclear with some cytoplasmic staining was observed. Staining was performed by Histowiz.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of LMO2 in THP-1 cells. Samples were incubated with LMO2 monoclonal antibody (Product # MA1-46422) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (Product # 35503). Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometry of LMO2 in Ramos cells. Samples were incubated with LMO2 monoclonal antibody (Product # MA1-46422) using a dilution of 1.0 µg/mL for 30 minutes at room temperature followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (Product # 35503). Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.