PA5-96911

antibody from Invitrogen Antibodies

Targeting: MBNL1 EXP, EXP35, EXP40, EXP42, KIAA0428, MBNL

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-96911

Provider

Invitrogen Antibodies

Product name

MBNL1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MAVSVTPIRD TKWLTLEVCR EFQRGTCSRP DTECKFAHPS KSCQVENGRV IACFDSLKGR CSRENCKYLH PPPHLKTQLE INGRNNLIQQ KNMAMLAQQM QLANAMMPGA PLQPVPMFSV APSLATNASA AAFNPYLGPV SPSLVPAEIL PTAPMLVTGN PGVPVPAAAA AAAQKLMRTD RLEVCREYQR GNCNRGENDC RFAHPADSTM IDTNDNTVTV CMDYIKGRCS REKCKYFHPP AHLQAKIKAA QYQVNQAAAA QAAATAAAMG IPQAVLPPLP KRPALEKTNG ATAVFNTGIF QYQQALANMQ LQQHTAFLPP GSILCMTPAT SVVPMVHGAT PATVSAATTS ATSVPFAATA TANQIPIISA EHLTSHKYVT QM; Positive Samples: Jurkat, HepG2, MCF7; Cellular Location: Cytoplasm, Cytoplasmic granule, Nucleus

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.71 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MBNL1 in extracts of various cell lines using MBNL1 Polyclonal Antibody (Product # PA5-96911) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry-Immunofluorescence analysis of MBNL1 was performed in MCF7 cells using MBNL1 Polyclonal Antibody (Product # PA5-96911).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of MBNL1 in paraffin-embedded rat heart using MBNL1 Polyclonal Antibody (Product # PA5-96911) at a dilution of 1:100.