MA5-17260

antibody from Invitrogen Antibodies

Targeting: COIL CLN80, p80-coilin

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

MA5-17260

Provider

Invitrogen Antibodies

Product name

Anti-Coilin Monoclonal Antibody (1H10)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

A suggested positive control for this product is HeLa cell lysate.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

1H10

Vial size

100 µL

Concentration

1 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Coilin using anti-Coilin monoclonal antibody (Product # MA5-17260) on (Lane 1) HeLa Cell lysate, (Lane 2) SK-N-MC Cell lysate, (Lane 3) K562 Cell lysate, (Lane 4) A431 Cell lysate, (Lane 5) HepG2 Cell lysate, (Lane 6) SK-BR3 Cell lysate and (Lane 7) Ramos Cell lysate.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Coilin was achieved by transfecting HeLa with Coilin specific siRNAs (Silencer® select Product # s15663, s15662). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Coilin knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with Coilin Monoclonal Antibody (1H10) (Product # MA5-17260, 0.5ug/ml ) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Coilin. An uncharacterized band at ~25 kDa was also observed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-Coilin Monoclonal Antibody (1H10) (Product # MA5-17260) and a 63kDa band corresponding to Coilin was observed across cell lines and tissue tested. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), Jurkat (Lane 2), Hep G2 (Lane 3), A549 (Lane 4), A-431 (Lane 5), K-562 (Lane 6), Reh (Lane 7), U-2 OS (Lane 8), MCF7 (Lane 9), Rat Testis (Lane 10) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (0.5 ug/mL) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177,1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005). An uncharacterized band was observed at ~25 kDa.