Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX112570 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX112570, RRID:AB_11177315
- Product name
- Coilin antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: 293T B: A431 C: HeLa D: HepG2 7.5% SDS PAGE GTX112570 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Coilin antibody detects Coilin protein by western blot analysis.A. 30 ?g 293T nuclear extract7.5 % SDS-PAGECoilin antibody (GTX112570) dilution: 1:1000
- Validation comment
- WB
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Coilin antibody detects Coilin protein by Western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg 293T nuclear extract7.5 % SDS-PAGECoilin antibody (GTX112570) dilution: 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Coilin antibody detects Coilin protein by Western blot analysis.A. 30 £gg HepG2 whole cell extract B. 30 £gg HepG2 nuclear extract7.5 % SDS-PAGECoilin antibody (GTX112570) dilution: 1:1000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using Coilin(GTX112570) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:500.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of Coilin protein from 293T whole cell extracts using 5 £gg of Coilin antibody (GTX112570).Western blot analysis was performed using Coilin antibody (GTX112570).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Coilin antibody detects Coilin protein at nucleus in human cervical cancer by immunohistochemical analysis. Sample: Paraffin-embedded human cervical cancer. Coilin antibody (GTX112570) diluted at 1:500.
