H00005764-M01

antibody from Novus Biologicals

Targeting: PTN HBGF8, HBNF, NEGF1

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Proximity ligation assay (Data presented on provider website)

Antibody Data

Product number

H00005764-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00005764-M01, RRID:AB_2174528

Product name

Mouse Monoclonal Pleiotrophin/PTN Antibody

Antibody type

Monoclonal

Description

IgG purified. PTN - pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)

Reactivity

Human, Rat, Chicken/Avian

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Expression of the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta in the serum, cartilage and subchondral bone of patients with osteoarthritis.

Kaspiris A, Mikelis C, Heroult M, Khaldi L, Grivas TB, Kouvaras I, Dangas S, Vasiliadis E, Lioté F, Courty J, Papadimitriou E
Joint bone spine 2013 Jul;80(4):407-13

---

Interplay between αvβ3 integrin and nucleolin regulates human endothelial and glioma cell migration.

Koutsioumpa M, Polytarchou C, Courty J, Zhang Y, Kieffer N, Mikelis C, Skandalis SS, Hellman U, Iliopoulos D, Papadimitriou E
The Journal of biological chemistry 2013 Jan 4;288(1):343-54

---

Implications of pleiotrophin in human PC3 prostate cancer cell growth in vivo.

Tsirmoula S, Dimas K, Hatziapostolou M, Lamprou M, Ravazoula P, Papadimitriou E
Cancer science 2012 Oct;103(10):1826-32

---

Pleiotrophin promotes microglia proliferation and secretion of neurotrophic factors by activating extracellular signal-regulated kinase 1/2 pathway.

Miao J, Ding M, Zhang A, Xiao Z, Qi W, Luo N, Di W, Tao Y, Fang Y
Neuroscience research 2012 Dec;74(3-4):269-76

---

A peptide corresponding to the C-terminal region of pleiotrophin inhibits angiogenesis in vivo and in vitro.

Mikelis C, Lamprou M, Koutsioumpa M, Koutsioubas AG, Spyranti Z, Zompra AA, Spiliopoulos N, Vradis AA, Katsoris P, Spyroulias GA, Cordopatis P, Courty J, Papadimitriou E
Journal of cellular biochemistry 2011 Jun;112(6):1532-43

---

Integrin alpha(v)beta(3) is a pleiotrophin receptor required for pleiotrophin-induced endothelial cell migration through receptor protein tyrosine phosphatase beta/zeta.

Mikelis C, Sfaelou E, Koutsioumpa M, Kieffer N, Papadimitriou E
FASEB journal : official publication of the Federation of American Societies for Experimental Biology 2009 May;23(5):1459-69

---

Aprotinin stimulates angiogenesis and human endothelial cell migration through the growth factor pleiotrophin and its receptor protein tyrosine phosphatase beta/zeta.

Koutsioumpa M, Hatziapostolou M, Mikelis C, Koolwijk P, Papadimitriou E
European journal of pharmacology 2009 Jan 14;602(2-3):245-9

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of PTN expression in transfected 293T cell line by PTN monoclonal antibody (M01), clone 5C3.Lane 1: PTN transfected lysate(18.9 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - PTN monoclonal antibody (M01), clone 5C3 Analysis of PTN expression in Hela S3 NE.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: Pleiotrophin/PTN Antibody (5C3) [H00005764-M01] - Analysis of monoclonal antibody to PTN on formalin-fixed paraffin-embedded human cerebral cortex. Antibody concentration 3 ug/ml.