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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- M00115 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-IRF7 Rabbit Monoclonal Antibody
- Antibody type
- Monoclonal
- Description
- Monoclonal antibody for IRF 7/IRF7 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: Flow Cytometry, IP, IF, IHC, ICC, WB. Reactive species: Human, Mouse, Rat IRF 7/IRF7 information: Molecular Weight: 54278 MW; Subcellular Localization: Nucleus. Cytoplasm. The phosphorylated and active form accumulates selectively in the nucleus; Tissue Specificity: Expressed predominantly in spleen, thymus and peripheral blood leukocytes.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antibody clone number
- FAH-9
- Vial size
- 100ug/vial
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
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- Experimental details
- Western blot analysis of IRF7 expression in Jurkat cell lysate (M00115). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IRF7 monoclonal antibody (Catalog # M00115) overnight at 4°C; then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for IRF7
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