AB-244-NA

antibody from Novus Biologicals

Targeting: TGFB3 ARVD, ARVD1

Western blot (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-244-NA

Provider

Novus Biologicals

Product name

Goat Polyclonal TGF-beta 3 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects TGF-beta 3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant amphibian TGF-beta 5 is observed and less than 5% cross-reactivity with recombinant human (rh) TGF-beta 1, rhTGF-beta 2 and rhLAP (TGF-beta 1) is observed.

Host

Goat

Conjugate

Unconjugated

Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.

References

Submitted references

Matrix metalloproteinase-25 has a functional role in mouse secondary palate development and is a downstream target of TGF-β3.

Brown GD, Nazarali AJ
BMC developmental biology 2010 Sep 1;10:93

---

Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.

Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
American journal of respiratory cell and molecular biology 2008 Jan;38(1):95-104

---

Isoform specificity of commercially-available anti-TGF-beta antibodies.

Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93

---

Isoform specificity of commercially-available anti-TGF-beta antibodies.

Mozes MM, Hodics T, Kopp JB
Journal of immunological methods 1999 May 27;225(1-2):87-93

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TGF-beta 3 by Simple Western^TM. Simple Western lane view shows lysates of human heart tissue and human breast cancer tissue, loaded at 0.2 mg/mL. A specific band was detected for TGF-beta 3 at approximately 64 kDa (as indicated) using 50 µg/mL of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Detection of Human TGF-beta 3 by Western Blot. Western blot shows lysates of human heart tissue and human breast cancer tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TGF-beta 3 at approximately 67 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Blocking/Neutralizing

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

TGF-beta 3 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 3 Antibody. Recombinant Human TGF-beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-TGF-beta 3 Polyclonal Antibody (Catalog # AB-244-NA). The ND₅₀ is typically 1-3 µg/mL.