MAB243-100

antibody from R&D Systems

Targeting: TGFB3 ARVD, ARVD1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB243-100

Provider

R&D Systems

Product name

TGF-beta 3 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from ascites. Detects TGF-beta 3 from multiple species in direct ELISAs and Western blots. In Western blots, less than 25% cross-reactivity with recombinant human (rh) TGF-beta 1.2 and rhTGF-beta 2 is observed, and less than 2% cross-reactivity with recombinant amphibian TGF-beta 5 and recombinant human TGF-beta 1 is observed. Neutralizes the biological activity of TGF-beta 3 but not TGF-beta 1, TGF-beta 2, or TGF-beta 5.

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

P10600


Isotype

IgG

Antibody clone number

20724

Vial size

100 ug

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

TGF-β3-expressing CD4+CD25(-)LAG3+ regulatory T cells control humoral immune responses.

Okamura T, Sumitomo S, Morita K, Iwasaki Y, Inoue M, Nakachi S, Komai T, Shoda H, Miyazaki J, Fujio K, Yamamoto K
Nature communications 2015 Feb 19;6:6329

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An autocrine TGF-beta/ZEB/miR-200 signaling network regulates establishment and maintenance of epithelial-mesenchymal transition.

Gregory PA, Bracken CP, Smith E, Bert AG, Wright JA, Roslan S, Morris M, Wyatt L, Farshid G, Lim YY, Lindeman GJ, Shannon MF, Drew PA, Khew-Goodall Y, Goodall GJ
Molecular biology of the cell 2011 May 15;22(10):1686-98

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Differential versican isoforms and aggrecan expression in the chicken embryo aorta.

Arciniegas E, Neves CY, Candelle D, Parada D
The anatomical record. Part A, Discoveries in molecular, cellular, and evolutionary biology 2004 Jul;279(1):592-600

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Th2 cytokine regulation of type I collagen gel contraction mediated by human lung mesenchymal cells.

Liu X, Kohyama T, Wang H, Zhu YK, Wen FQ, Kim HJ, Romberger DJ, Rennard SI
American journal of physiology. Lung cellular and molecular physiology 2002 May;282(5):L1049-56

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TGF-beta 3 in Human Breast Cancer Tissue. TGF-beta 3 was detected in immersion fixed paraffin-embedded sections of human breast cancer tissue using Mouse Anti-TGF-beta 3 Monoclonal Antibody (Catalog # MAB243) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in cancer cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

TGF-beta 3 Inhibition of IL-4-dependent Cell Proliferation and Neutralization by TGF-beta 3 Antibody. Recombinant Human TGF-beta 3 (Catalog # 243-B3) inhibits Recombinant Mouse IL-4 (Catalog # 404-ML) induced proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Mouse IL-4 (7.5 ng/mL) activity elicited by Recombinant Human TGF-beta 3 (0.1 ng/mL) is neutralized (green line) by increasing concen-trations of Mouse Anti-TGF-beta 3 Monoclonal Antibody (Catalog # MAB243). The ND₅₀ is typically 0.1-0.3 µg/mL.