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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-97064 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TGF beta-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: STCTTLDFGH IKKKRVEAIR GQILSKLRLT SPPEPTVMTH VPYQVLALYN STRELLEEMH GEREEGCTQE NTESEYYAKE IHKFDMIQGL AEHNELAVCP KGITSKVFRF NVSSVEKNRT NLFRAEFRVL RVPNPSSKRN EQRIELFQIL RPDEHIAKQR YIGGKNLPTR GTAEWLSFDV TDTVREWLLR RESNLGLEIS IHCPCHTFQP NGDILENIHE VMEIKFKGVD NEDDHGRGDL GRLKKQKDHH NPHLILMMIP PHRLDNPGQG GQRKKR; Positive Samples: BT-474, K-937, Mouse lung, Mouse skeletal muscle, Mouse heart, Rat lung, Rat heart
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 2.11 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of TGF beta-3 in extracts of various cell lines using TGF beta-3 Polyclonal Antibody (Product # PA5-97064) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of TGF beta-3 was performed in HeLa cells using TGF beta-3 Polyclonal Antibody (Product # PA5-97064) at a dilution of 1:100. Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TGF beta-3 in paraffin-embedded mouse lung using TGF beta-3 Polyclonal Antibody (Product # PA5-97064) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of TGF beta-3 in paraffin-embedded mouse lung using TGF beta-3 Polyclonal Antibody (Product # PA5-97064) at a dilution of 1:100.
