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LearnGTX129237
antibody from GeneTex
Targeting: TCF3
bHLHb21, E2A, E47, ITF1, MGC129647, MGC129648, p75, VDIR
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- GTX129237 - Provider product page
- Provider
- GeneTex
- Product name
- TCF3 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCF3 antibody detects TCF3 protein by western blot analysis.A. 30 £gg Jurkat whole cell extractB. 30 £gg Raji whole cell extractC. 30 £gg NCI-H929 whole cell extract7.5 % SDS-PAGETCF3 antibody (GTX129237) dilution: 1:2500
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCF3 antibody detects TCF3 protein by western blot analysis.A. 30 £gg NCI-H929 whole cell extractB. 30 £gg NCI-H929 nuclear extract7.5 % SDS-PAGETCF3 antibody (GTX129237) dilution: 1:3000
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCF3 antibody detects TCF3 protein at nucleus by immunofluorescent analysis.Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: TCF3 protein stained by TCF3 antibody (GTX129237) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- TCF3 antibody immunoprecipitates TCF3 protein in IP experiments.IP samples: Jurkat whole cell extractA. 50 £gg Jurkat whole cell extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of TCF3 protein by 4 £gg TCF3 antibody (GTX129237)7.5 % SDS-PAGEThe immunoprecipitated TCF3 protein was detected by TCF3 antibody (GTX129237) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
