- PA5-78190 - Invitrogen Antibodies TCF3 antibody

No submitted references: Submit reference

No comments: Submit comment

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TCF3 in A) NCI-H929 whole cell extract, B) NCI-H929 nuclear extract using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with TCF3 polyclonal antibody (Product # PA5-78190) using a dilution of 1:3000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of TCF3 in A) Jurkat whole cell extract, B) Raji whole cell extract, C) NCI-H929 whole cell extract using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with TCF3 polyclonal antibody (Product # PA5-78190) using a dilution of 1:2500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TCF3 Polyclonal Antibody detects TCF3 protein by western blot analysis. A. 30 µg Jurkat whole cell extract. B. 30 µg Raji whole cell extract. C. 30 µg NCI-H929 whole cell extract.7.5 % SDS-PAGE. TCF3 Polyclonal Antibody (Product # PA5-78190) dilution: 1:2,500.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TCF3 Polyclonal Antibody detects TCF3 protein by western blot analysis. A. 30 µg NCI-H929 whole cell extract. B. 30 µg NCI-H929 nuclear extract.7.5 % SDS-PAGE. TCF3 Polyclonal Antibody (Product # PA5-78190) dilution: 1:3,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot was performed using Anti-TCF3 Polyclonal Antibody (Product # PA5-78190) and a 68 kDa band corresponding to TCF-3 was observed in all the tested cell lines and tissues, except Mouse Brain and Rat Brain. Whole cell lysates (30 µg lysate) of HCT 116 (Lane 1), MCF-7 (Lane 2), HeLa (Lane 3), OVCAR-3 (Lane 4), Raji (Lane 5), tissue lysates (30 µg lysates) of Mouse Brain (Lane 6), Mouse Liver (Lane 7), Rat Brain (Lane 8) and Rat Liver (Lane 9) were electrophoresed using NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blots were probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TCF3 Polyclonal Antibody detects TCF3 protein at nucleus by immunofluorescent analysis. Sample: MCF-7 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: TCF3 protein stained by TCF3 Polyclonal Antibody (Product # PA5-78190) diluted at 1:500. Blue: Hoechst 33342 staining.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: TCF3 Polyclonal Antibody immunoprecipitates TCF3 protein in IP experiments. IP samples: Jurkat whole cell extract. A. 50 µg Jurkat whole cell extract. B. Control with 4 µg of preimmune Rabbit IgG. C. Immunoprecipitation of TCF3 protein by 4 µg TCF3 Polyclonal Antibody (Product # PA5-78190). 7.5 % SDS-PAGE. The immunoprecipitated TCF3 protein was detected by TCF3 Polyclonal Antibody (Product # PA5-78190) diluted at 1:500.

PA5-78190