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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [7]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- sc-440 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-440, RRID:AB_673599
- Product name
- Anti-CREM
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Rabbit
Submitted references Use of bicistronic vectors in combination with flow cytometry to screen for effective small interfering RNA target sequences.
DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding.
Jmjd1a demethylase-regulated histone modification is essential for cAMP-response element modulator-regulated gene expression and spermatogenesis.
Krüppel-like factor 4, a "pluripotency transcription factor" highly expressed in male postmeiotic germ cells, is dispensable for spermatogenesis in the mouse.
Regulation of murine lactate dehydrogenase C (Ldhc) gene expression.
The role of transcription factors cyclic-AMP responsive element modulator (CREM) and inducible cyclic-AMP early repressor (ICER) in epileptogenesis.
cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis.
Kamio N, Hirai H, Ashihara E, Tenen DG, Maekawa T, Imanishi J
Biochemical and biophysical research communications 2010 Mar 12;393(3):498-503
Biochemical and biophysical research communications 2010 Mar 12;393(3):498-503
DNA cytosine methylation in the bovine leukemia virus promoter is associated with latency in a lymphoma-derived B-cell line: potential involvement of direct inhibition of cAMP-responsive element (CRE)-binding protein/CRE modulator/activation transcription factor binding.
Pierard V, Guiguen A, Colin L, Wijmeersch G, Vanhulle C, Van Driessche B, Dekoninck A, Blazkova J, Cardona C, Merimi M, Vierendeel V, Calomme C, Nguyên TL, Nuttinck M, Twizere JC, Kettmann R, Portetelle D, Burny A, Hirsch I, Rohr O, Van Lint C
The Journal of biological chemistry 2010 Jun 18;285(25):19434-49
The Journal of biological chemistry 2010 Jun 18;285(25):19434-49
Jmjd1a demethylase-regulated histone modification is essential for cAMP-response element modulator-regulated gene expression and spermatogenesis.
Liu Z, Zhou S, Liao L, Chen X, Meistrich M, Xu J
The Journal of biological chemistry 2010 Jan 22;285(4):2758-70
The Journal of biological chemistry 2010 Jan 22;285(4):2758-70
Krüppel-like factor 4, a "pluripotency transcription factor" highly expressed in male postmeiotic germ cells, is dispensable for spermatogenesis in the mouse.
Godmann M, Gashaw I, Katz JP, Nagy A, Kaestner KH, Behr R
Mechanisms of development 2009 Aug-Sep;126(8-9):650-64
Mechanisms of development 2009 Aug-Sep;126(8-9):650-64
Regulation of murine lactate dehydrogenase C (Ldhc) gene expression.
Tang H, Kung A, Goldberg E
Biology of reproduction 2008 Mar;78(3):455-61
Biology of reproduction 2008 Mar;78(3):455-61
The role of transcription factors cyclic-AMP responsive element modulator (CREM) and inducible cyclic-AMP early repressor (ICER) in epileptogenesis.
Porter BE, Lund IV, Varodayan FP, Wallace RW, Blendy JA
Neuroscience 2008 Mar 27;152(3):829-36
Neuroscience 2008 Mar 27;152(3):829-36
cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis.
Somboonthum P, Ohta H, Yamada S, Onishi M, Ike A, Nishimune Y, Nozaki M
Nucleic acids research 2005;33(10):3401-11
Nucleic acids research 2005;33(10):3401-11
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:3000
- Lane 1
- Marker [kDa]: 250, 130, 95, 72, 55, 36, 28, 17, 11
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 28
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human testis shows strong nuclear positivity in spermatids.
- Validation comment
- Two independent antibodies targeting one protein yielding similar staining patterns. Staining pattern consistent with experimental and/or bioinformatic data.
