PA5-17516

antibody from Invitrogen Antibodies

Targeting: PPP2R1A PP2A-Aalpha, PP2AA, PR65A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-17516

Provider

Invitrogen Antibodies

Product name

PPP2R1A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

It is not recommended to aliquot this antibody.

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus PPP2CA siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A549 cells were lysed 72 hours after transfection. Cells were transfected with Transfection Reagent alone (Lane 1), 100nM ON-TARGETplus siCONTROL Non-Targeting Pool (Lane 2), or 100nM ONTARGETplus PPP2CA siRNA (Lane 3). Western blot data for GAPDH is included as a control for equal protein loading.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of PP2A A Subunit in paraformaldehyde fixed HeLa cells using a PP2A A Subunit polyclonal antibody (Product # PA5-17516).

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow cytometric analysis of PP2A A Subunit in NIH/3T3 cells using a PP2A A Subunit polyclonal antibody (Product # PA5-17516) (blue) compared to a nonspecifc negative control antibody (red).