MA1-20112

antibody from Invitrogen Antibodies

Targeting: TNNI3 CMD2A, CMH7, TNNC1

Provider product page for MA1-20112

Western blot

ELISA

Immunoprecipitation

Immunohistochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [8]
Comments [0]
Validations
Western blot [3]
ELISA [1]
Other assay [1]

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Product number: MA1-20112 - Provider product page
Provider: Invitrogen Antibodies
Product name: Cardiac Troponin I Monoclonal Antibody (16A11)
Antibody type: Monoclonal
Antigen: Other
Description: MA1-20112 detects cardiac troponin 1 in human, mouse, rat, bovine, feline, canine, goat, porcine and rabbit samples.
Reactivity: Human, Mouse, Rat, Bovine, Canine, Feline, Goat, Porcine, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: 16A11
Vial size: 200 µg
Concentration: 5.5 mg/mL
Storage: 4° C

TNNI3 protein structure - MA1-20112 shown in red.

Submitted references Boosting Akt Pathway by Rupatadine Modulates Th17/Tregs Balance for Attenuation of Isoproterenol-Induced Heart Failure in Rats.

Ahmed LA, Mohamed AF, Abd El-Haleim EA, El-Tanbouly DM
Frontiers in pharmacology 2021;12:651150

ERRγ enhances cardiac maturation with T-tubule formation in human iPSC-derived cardiomyocytes.

Miki K, Deguchi K, Nakanishi-Koakutsu M, Lucena-Cacace A, Kondo S, Fujiwara Y, Hatani T, Sasaki M, Naka Y, Okubo C, Narita M, Takei I, Napier SC, Sugo T, Imaichi S, Monjo T, Ando T, Tamura N, Imahashi K, Nishimoto T, Yoshida Y
Nature communications 2021 Jun 21;12(1):3596

Hydrogen-rich saline alleviates inflammation and apoptosis in myocardial I/R injury via PINK-mediated autophagy.

Yao L, Chen H, Wu Q, Xie K
International journal of molecular medicine 2019 Sep;44(3):1048-1062

Spliceosomal protein eftud2 mutation leads to p53-dependent apoptosis in zebrafish neural progenitors.

Lei L, Yan SY, Yang R, Chen JY, Li Y, Bu Y, Chang N, Zhou Q, Zhu X, Li CY, Xiong JW
Nucleic acids research 2017 Apr 7;45(6):3422-3436

Sonic hedgehog controls enteric nervous system development by patterning the extracellular matrix.

Nagy N, Barad C, Graham HK, Hotta R, Cheng LS, Fejszak N, Goldstein AM
Development (Cambridge, England) 2016 Jan 15;143(2):264-75

Increased radial glia quiescence, decreased reactivation upon injury and unaltered neuroblast behavior underlie decreased neurogenesis in the aging zebrafish telencephalon.

Edelmann K, Glashauser L, Sprungala S, Hesl B, Fritschle M, Ninkovic J, Godinho L, Chapouton P
The Journal of comparative neurology 2013 Sep 1;521(13):3099-115

The cellular composition of neurogenic periventricular zones in the adult zebrafish forebrain.

Lindsey BW, Darabie A, Tropepe V
The Journal of comparative neurology 2012 Jul 1;520(10):2275-316

Neuronal and glial localization of alpha(2A)-adrenoceptors in the adult zebrafish (Danio rerio) brain.

Ampatzis K, Kentouri M, Dermon CR
The Journal of comparative neurology 2008 May 1;508(1):72-93

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of Cardiac Troponin I in 3 ng (Lane 1) 10 ng (Lane 2) 30 ng (Lane 3) and 100 ng (Lane 4) cTNI using a Cardiac Troponin I monoclonal antibody (Product # MA1-20112). Detection was performed using an HRP-conjugated goat anti-mouse IgG antibody and diaminobenzidine substrate.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Heart (Lane 1), Rat Heart (Lane 2), Mouse Liver (Lane 3), Mouse Skeletal Muscle (Lane 4) and Mouse Brain (Lane 5). The blot was probed with Anti-Cardiac Troponin I Monoclonal Antibody (16A11) (Product # MA1-20112, 1:500 dilution) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 µg/ml, 1:4000 dilution). A 24 kDa band corresponding to Cardiac Troponin I was detected only in Mouse and Rat Heart which are reported to be positive for Cardiac Troponin I expression.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Detection of cardiac troponin I (cTnI) by Western blotting. After SDS-PAGE cTnI was transferred to a nitrocellulose membrane and probed by Cardiac Troponin I Monoclonal Antibody (16A11) (Product # MA1-20112) and then by a HRP-conjugated Goat anti-Mouse IgG (H+L) Secondary Antibody. Substrate - diaminobenzidine. Lane 1: 3 ng cTnI; Lane 2: 10 ng cTnI; Lane 3: 30 ng cTnI; Lane 4: 100 ng cTnI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Cardiac troponin I measurements using Cardiac Troponin I Monoclonal Antibody (16A11) (Product # MA1-20112) in a sandwich ELISA to determine the total cardiac troponin I in serial blood samples from AMI patient.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: FIGURE 5 Effect of RUP with or without wortmannin on ISO-induced changes in myocardial content of (A) atrogin-1 as well as the protein expression of (B) troponin I and (C) troponin T. Each value represents the mean of six experiments and error bars represent SD. Statistical analysis was done using One way ANOVA followed by Tukey's post-hoc test where a p