Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- MAB6887 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Cardiac Troponin I Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human Cardiac Troponin I in direct ELISAs and Western blots.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P19429
- Isotype
- IgG
- Antibody clone number
- 679013
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Wnt inhibition promotes vascular specification of embryonic cardiac progenitors.
Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes.
Reichman DE, Park L, Man L, Redmond D, Chao K, Harvey RP, Taketo MM, Rosenwaks Z, James D
Development (Cambridge, England) 2018 Jan 8;145(1)
Development (Cambridge, England) 2018 Jan 8;145(1)
Human embryonic stem cell derived mesenchymal progenitors express cardiac markers but do not form contractile cardiomyocytes.
Raynaud CM, Halabi N, Elliott DA, Pasquier J, Elefanty AG, Stanley EG, Rafii A
PloS one 2013;8(1):e54524
PloS one 2013;8(1):e54524
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human Cardiac Troponin I by Western Blot. Western blot shows lysates of human heart tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cardiac Troponin I Monoclonal Antibody (Catalog # MAB6887) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Cardiac Troponin I at approximately 24-29 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.