Antibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
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- Product number
- PA1-340 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP1A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Purifed from natural sources
- Description
- PA1-340 detects cytochrome P450 (P450) 1A1 from human cells. This antibody detects, to a lesser extent, other P450 isoforms.
Submitted references Detoxification: a novel function of BRCA1 in tumor suppression?
A novel in vitro pancreatic carcinogenesis model.
Identification through microarray gene expression analysis of cellular responses to benzo(a)pyrene and its diol-epoxide that are dependent or independent of p53.
Kang HJ, Hong YB, Kim HJ, Rodriguez OC, Nath RG, Tilli EM, Albanese C, Chung FL, Kwon SH, Bae I
Toxicological sciences : an official journal of the Society of Toxicology 2011 Jul;122(1):26-37
Toxicological sciences : an official journal of the Society of Toxicology 2011 Jul;122(1):26-37
A novel in vitro pancreatic carcinogenesis model.
Kang HJ, Hong YB, Kim HJ, Yi YW, Nath RG, Chang YS, Cho HC, Bae I
Toxicology letters 2011 Apr 10;202(1):15-22
Toxicology letters 2011 Apr 10;202(1):15-22
Identification through microarray gene expression analysis of cellular responses to benzo(a)pyrene and its diol-epoxide that are dependent or independent of p53.
Hockley SL, Arlt VM, Jahnke G, Hartwig A, Giddings I, Phillips DH
Carcinogenesis 2008 Jan;29(1):202-10
Carcinogenesis 2008 Jan;29(1):202-10
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot detection of cytochrome P450 1A1 from MCF-7 cell extract using Product # PA1-340.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis was performed on whole cell extracts (30 µg lysate) of Hep G2 (Lane 1), LNCaP (Lane 2), K562 (Lane 3), CaCo-2 (Lane 4) and HeLa (Lane 5). The blots were probed with Anti-Cytochrome P450 1A1 Rabbit Polyclonal Antibody (Product # PA1-340, 1:100-1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate (Product # G-21234, 1:5000 dilution). A 58 kDa band corresponding to Cytochrome P450 1A1 was observed across cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0301BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5 % skimmed milk. Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of Cytochrome P450 1A1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Cytochrome P450 1A1 Rabbit Polyclonal Antibody (Product # PA1-340) at 1:250 dilution in0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d is a merged image showing cytoplasmic localization. Panel e is a no primary antibody control. The images were captured at 60X magnification.