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LearnH00004171-M01
antibody from Novus Biologicals
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00004171-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00004171-M01, RRID:AB_540360
- Product name
- Mouse Monoclonal MCM2 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. MCM2 - MCM2 minichromosome maintenance deficient 2, mitotin (S. cerevisiae)
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Submitted references Profiling DNA damage response following mitotic perturbations.
Pro-tumorigenic effects of miR-31 loss in mesothelioma.
S Pedersen R, Karemore G, Gudjonsson T, Rask MB, Neumann B, Hériché JK, Pepperkok R, Ellenberg J, Gerlich DW, Lukas J, Lukas C
Nature communications 2016 Dec 15;7:13887
Nature communications 2016 Dec 15;7:13887
Pro-tumorigenic effects of miR-31 loss in mesothelioma.
Ivanov SV, Goparaju CM, Lopez P, Zavadil J, Toren-Haritan G, Rosenwald S, Hoshen M, Chajut A, Cohen D, Pass HI
The Journal of biological chemistry 2010 Jul 23;285(30):22809-17
The Journal of biological chemistry 2010 Jul 23;285(30):22809-17
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 expression in transfected 293T cell line by MCM2 monoclonal antibody (M01), clone 6A8.Lane 1: MCM2 transfected lysate(101.9 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MCM2 Antibody (6A8) [H00004171-M01] - MCM2 monoclonal antibody (M01), clone 6A8 Analysis of MCM2 expression in Hela S3 NE.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: MCM2 Antibody (6A8) [H00004171-M01] - Detection limit for recombinant GST tagged MCM2 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of monoclonal antibody to MCM2 on HeLa cell. Antibody concentration 10 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MCM2 Antibody (6A8) [H00004171-M01] - Analysis of MCM2 transfected lysate using anti-MCM2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MCM2 MaxPab rabbit polyclonal antibody.
