PA5-86175
antibody from Invitrogen Antibodies
Targeting: MCM2
BM28, CCNL1, cdc19, CDCL1, D3S3194, DFNA70, KIAA0030
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Other assay [1]
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Validation data
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- Product number
- PA5-86175 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- MCM2 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCM2 in Lane 1: SK-OVCAR3 whole cell lysate (40 µg), Lane 2: HCT116 whole cell lysate (40 µg), Lane 3: CT26 whole cell lysate (40 µg), Lane 4: rat testis tissue lysate (40 µg). Sample was incubated with MCM2 polyclonal antibody (Product # PA5-86175) using a 1:500 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of MCM2 in Lane 1: SK-OVCAR3 whole cell lysate (40 µg), Lane 2: HCT116 whole cell lysate (40 µg), Lane 3: CT26 whole cell lysate (40 µg), Lane 4: rat testis tissue lysate (40 µg). Sample was incubated with MCM2 polyclonal antibody (Product # PA5-86175) using a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of MCM2 in paraffin-embedded human breast carcinoma tissue. Sample was incubated with polyclonal MCM2 antibody (Product # PA5-86175) using a 1:100 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of MCM2 in HEK293T cell lysates (Lane 2 and Lane 3) and 30% input (Lane 1). Sample was incubated with MCM2 polyclonal antibody (Product # PA5-86175) Input: supernatant (arrow up) and deposition (arrow down).