Antibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Immunohistochemistry [1]
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Validation data
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- Product number
- M00297-2 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Fos Monoclonal Antibody (8B5)
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal 8B5 antibody for FOS detection. Tested positive for ELISA, IHC, WB in Human, Mouse, Rat
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Antibody clone number
- 8B5
- Vial size
- 100µg
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- -20°C
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- IHC analysis of FOS using anti-FOS antibody (M00297-2).FOS was detected in paraffin-embedded section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-FOS Antibody (M00297-2) overnight at 4°C. Biotinylated goat anti Mouse IgG antibody was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1021) with DAB as the chromogen.
- Additional image