MA3-217

antibody from Invitrogen Antibodies

Targeting: TSHR LGR3

Western blot (Recommended by provider)

ELISA (Recommended by provider)

Immunocytochemistry (Recommended by provider)

Antibody Data

Product number

MA3-217

Provider

Invitrogen Antibodies

Product name

Anti-TSH Receptor Monoclonal Antibody (28)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

MA3-218 detects both glycosylated and nonglycosylated thyroid stimulating hormone receptor (TSHR) from porcine tissues as well as recombinant human TSHR. MA3-218 has been successfully used in Western blot, immunofluorescence, and ELISA procedures. By Western blot, this antibody detects a 50 kDa and a 63 kDa protein representing unglycosylated and glycosylated TSHR respectively, in transfected Sf9 cell extract. By ELISA MA3-218 has been used in TSH competitive inhibition studies. The MA3-218 antigen is purified extracellular domain of recombinant human thyrotropin receptor. This antibody recognizes an epitope between amino acid residues 277-296 of human TSHR.

Reactivity

Human, Porcine

Host

Mouse

Isotype

IgG

Antibody clone number

28

Vial size

100 µL

Concentration

Lot Dependent

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Identification and functional characterization of a novel thyrotropin receptor mutation (V87L) in a Chinese woman with subclinical hypothyroidism.

Zhang HM, Zhou YQ, Dong Y, Su Q
Experimental and therapeutic medicine 2017 Jan;13(1):290-294

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Overexpression of thyroid hormone receptor beta1 is associated with thyrotropin receptor gene expression and proliferation in a human thyroid carcinoma cell line.

Chen ST, Shieh HY, Lin JD, Chang KS, Lin KH
The Journal of endocrinology 2000 May;165(2):379-89

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Differentiation of human orbital preadipocyte fibroblasts induces expression of functional thyrotropin receptor.

Valyasevi RW, Erickson DZ, Harteneck DA, Dutton CM, Heufelder AE, Jyonouchi SC, Bahn RS
The Journal of clinical endocrinology and metabolism 1999 Jul;84(7):2557-62

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Differential reactivities of recombinant glycosylated ectodomains of mouse and human thyrotropin receptors with patient autoantibodies.

Patibandla SA, Seetharamaiah GS, Dallas JS, Thotakura NR, Peake RL, Prabhakar BS
Endocrinology 1997 Apr;138(4):1559-66

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Generation and characterization of monoclonal antibodies to the human thyrotropin (TSH) receptor: antibodies can bind to discrete conformational or linear epitopes and block TSH binding.

Seetharamaiah GS, Wagle NM, Morris JC, Prabhakar BS
Endocrinology 1995 Jul;136(7):2817-24