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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunohistochemistry [2]
- Other assay [1]
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- Product number
- A302-497A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SPF45 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of mouse SPF45 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. A302-497A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SPF45 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SPF45 (Cat. No. A302-497A Lot2) used at a dilution of 1:500 (2µg/ml). Detection: DAB.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human SPF45 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SPF45 antibody A302-497A (lot A302-497A-2) used for IP at 6 µg per reaction. SPF45 was also immunoprecipitated by a previous lot of this antibody (lot A302-497A-1) and rabbit anti-SPF45 antibody A302-498A. For blotting immunoprecipitated SPF45, A302-497A-2 was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
