PAB21333

antibody from Abnova Corporation

Targeting: GUCY1B1 GC-S-beta-1, GC-SB3, GUC1B3, GUCY1B3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB21333

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB21333, RRID:AB_10965537

Product name

GUCY1B3 polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against recombinant GUCY1B3.

Antigen sequence

HALELLVIRNYGPEVWEDIKKEAQLDEEGQFLVRI
IYDDSKTYDLVAAASKVLNLNAGEILQMFGKMFFV
FCQESGYDTILRVLGSNVREFLQNLDALHDHLATI
YPGMRAPSFRCTDAEKGKGLILHYYSEREGLQDIV
IGII


Isotype

IgG

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western blot analysis of Lane 1: RT-4, Lane 2: U-251 MG, Lane 3: Human Plasma, Lane 4: Liver, Lane 5: Tonsil with GUCY1B3 polyclonal antibody (Cat # PAB21333) at 1:250-1:500 dilution.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line U-2 OS with GUCY1B3 polyclonal antibody (Cat # PAB21333) at 1-4 ug/mL dilution shows positivity in vesicles.

Validation comment

Immunofluorescence

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining of human colon with GUCY1B3 polyclonal antibody (Cat # PAB21333) strong cytoplasmic positivity in glandular cells at 1:20-1:50 dilution.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)