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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Flow cytometry [1]
- Other assay [2]
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- Product number
- 12-9919-42 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD178 (Fas Ligand) Monoclonal Antibody (NOK-1), PE, eBioscience™
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Isotype
- IgG
- Antibody clone number
- NOK-1
- Vial size
- 100 Tests
- Concentration
- 5 µL/Test
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Membrane Fas ligand kills human peripheral blood T lymphocytes, and soluble Fas ligand blocks the killing.
Expression of the functional soluble form of human fas ligand in activated lymphocytes.
Metalloproteinase-mediated release of human Fas ligand.
Suda T, Hashimoto H, Tanaka M, Ochi T, Nagata S
The Journal of experimental medicine 1997 Dec 15;186(12):2045-50
The Journal of experimental medicine 1997 Dec 15;186(12):2045-50
Expression of the functional soluble form of human fas ligand in activated lymphocytes.
Tanaka M, Suda T, Takahashi T, Nagata S
The EMBO journal 1995 Mar 15;14(6):1129-35
The EMBO journal 1995 Mar 15;14(6):1129-35
Metalloproteinase-mediated release of human Fas ligand.
Kayagaki N, Kawasaki A, Ebata T, Ohmoto H, Ikeda S, Inoue S, Yoshino K, Okumura K, Yagita H
The Journal of experimental medicine 1995 Dec 1;182(6):1777-83
The Journal of experimental medicine 1995 Dec 1;182(6):1777-83
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Surface staining of human FasL tranfected cells with Anti-Human CD178 (CD95 Ligand) PE. Appropriate isotype controls were used (open histogram). Total viable cells were used for analysis.
- Conjugate
- Yellow dye
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- NULL
- Conjugate
- Yellow dye
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- EVs secreted by Cal27 induce apoptosis in NK cells. a Cal27-WT and KO derived EVs were analyzed by flow cytometry for known NKG2D ligands MICA/B, ULBP2/5/6, ULPB3, and for TRAIL and FasL, proteins involved in intrinsic or extrinsic apoptosis pathway (green histograms). In blue is shown the isotype control. Representative of n = 2 independent experiments. b Flow cytometry CASP3/7 viability assay showing increasing NK cell death once exposed to EVs from Cal27 in a time course of 4-hours. The error bars represent +-SEM across n = 3 replicates. Two-way ANOVA and Bonferroni's post hoc test was performed to determine statistical significance. Representative of n = 3 independent experiments. c 24 hour time course viability assay quantified using the IncuCyte real-time imaging system. N = 5 images/well taken for each of three replicates. The error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by two-way ANOVA and Bonferroni's post hoc multiple comparison test. Representative of n = 2 independent experiments. d 4 hour cytotoxicity assay using NK cells as effectors against Cal27-WT or KO as target cells. Here we show how Cal27-derived EVs reduce the killing in Cal27-KO. Error bars represent +-SEM across n = 3 replicates. Statistical analysis was performed by one-way ANOVA and Bonferroni's post hoc test (ns, not significant). e antibodies blocking MICA/B, TRAIL, or a combination of the two (aMICA/B and aTRAIL) limit the inhibitory effect of EV on NK ce
- Conjugate
- Yellow dye
