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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- TA500353 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500353, RRID:AB_2133061
- Product name
- L1CAM mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)
- Antibody type
- Monoclonal
- Description
- L1CAM mouse monoclonal antibody, clone OTI1H3 (formerly 1H3)
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- L1CAM
- Isotype
- IgG
- Antibody clone number
- OTI1H3
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY L1CAM (RC211601, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-L1CAM.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-L1CAM monoclonal antibody.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-L1CAM mouse monoclonal antibody (TA500353) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY L1CAM(RC211601).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunoprecipitation of L1CAM by using TrueMab monoclonal anti-L1CAM antibody (Negative control: IP without adding anti-L1CAM antibody). For each experiment, 500ul of DDK tagged L1CAM overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of ant
- Validation comment
- IP
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells transfected with either RC211601 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-L1CAM antibody(TA500353), and then analyzed by flow cytometry.
- Validation comment
- FC
