GTX108273

antibody from GeneTex

Targeting: H2AZ1 H2A.Z, H2AFZ, H2AZ

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Chromatin Immunoprecipitation (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX108273

Provider

GeneTex

Proper citation

GeneTex Cat#GTX108273, RRID:AB_1950474

Product name

Histone H2A.Z antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Sample(30 £gg of whole cell lysate)A:MOLT4(GTX27912)B:Raji(GTX27908)12% SDS PAGEGTX108273 diluted at 1:3000

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunofluorescence analysis of paraformaldehyde-fixed A431, using Histone H2A.Z(GTX108273) antibody at 1:500 dilution.

Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein in IP experiments. IP Sample: 1000 ?g HeLa whole cell lysate/extract A. 50 £gg HeLa whole cell lysate/extract B. Control with 2 £gg of preimmune rabbit IgG C. Immunoprecipitation of Histone H2A.Z protein by 2 £gg of Histone H2A.Z antibody (GTX108273) 15% SDS-PAGE The immunoprecipitated Histone H2A.Z protein was detected by Histone H2A.Z antibody (GTX108273) diluted at 1:1000. EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent.

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2A.Z antibody detects Histone H2A.Z protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. Histone H2A.Z antibody (GTX108273) diluted at 1:1000.

Chromatin Immunoprecipitation

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Histone H2A.Z antibody immunoprecipitates Histone H2A.Z protein-DNA in ChIP experiments. ChIP Sample: HeLa whole cell lysate/extract A. 5 £gg preimmune rabbit IgG B. 5 £gg of Histone H2A.Z antibody (GTX108273) The precipitated DNA was detected by PCR with primer set targeting to GAPDH gene locus.