GTX102170

antibody from GeneTex

Targeting: FOXM1 FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP2, TGT3, trident

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Chromatin Immunoprecipitation (Data presented on provider website)

Antibody Data

Product number

GTX102170

Provider

GeneTex

Proper citation

GeneTex Cat#GTX102170, RRID:AB_10720162

Product name

FOXM1 antibody

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

References

Submitted references

Chromatin associated SETD3 negatively regulates VEGF expression.

Cohn O, Feldman M, Weil L, Kublanovsky M, Levy D
Scientific reports 2016 Nov 15;6:37115

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CDC20 maintains tumor initiating cells.

Xie Q, Wu Q, Mack SC, Yang K, Kim L, Hubert CG, Flavahan WA, Chu C, Bao S, Rich JN
Oncotarget 2015 May 30;6(15):13241-54

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PGRMC1 contributes to doxorubicin-induced chemoresistance in MES-SA uterine sarcoma.

Lin ST, May EW, Chang JF, Hu RY, Wang LH, Chan HL
Cellular and molecular life sciences : CMLS 2015 Jun;72(12):2395-409

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FOXM1 binds directly to non-consensus sequences in the human genome.

Sanders DA, Gormally MV, Marsico G, Beraldi D, Tannahill D, Balasubramanian S
Genome biology 2015 Jun 23;16(1):130

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Genome-wide mapping of FOXM1 binding reveals co-binding with estrogen receptor alpha in breast cancer cells.

Sanders DA, Ross-Innes CS, Beraldi D, Carroll JS, Balasubramanian S
Genome biology 2013 Jan 24;14(1):R6

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FOXM1 antibody detects FOXM1 protein by Western blot analysis.A. 30 µg 293T whole cell lysate/extractB. 30 µg A431 whole cell lysate/extractC. 30 µg HepG2 whole cell lysate/extract D. 30 µg A375 whole cell lysate/extract7.5 % SDS-PAGEFOXM1 antibody (GTX102170) dilution: 1:1000

Validation comment

WB

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

FOXM1 antibody detects FOXM1 protein by western blot analysis.A.30 ?g 293T whole cell lysate/extractB.30 ?g whole cell lysate/extract of human FOXM1-transfected 293T cells7.5% SDS-PAGEFOXM1 antibody (GTX102170) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Various whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with FOXM1 antibody (GTX102170) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.