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LearnNBP1-84671
antibody from Novus Biologicals
Targeting: FOXM1
FKHL16, HFH-11, HNF-3, INS-1, MPHOSPH2, MPP2, TGT3, trident
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [4]
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- Product number
- NBP1-84671 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-84671, RRID:AB_11017094
- Product name
- Rabbit Polyclonal FoxM1 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human FoxM1 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Honokiol is a FOXM1 antagonist.
FOXM1 contributes to treatment failure in acute myeloid leukemia.
Halasi M, Hitchinson B, Shah BN, Váraljai R, Khan I, Benevolenskaya EV, Gaponenko V, Arbiser JL, Gartel AL
Cell death & disease 2018 Jan 24;9(2):84
Cell death & disease 2018 Jan 24;9(2):84
FOXM1 contributes to treatment failure in acute myeloid leukemia.
Khan I, Halasi M, Patel A, Schultz R, Kalakota N, Chen YH, Aardsma N, Liu L, Crispino JD, Mahmud N, Frankfurt O, Gartel AL
JCI insight 2018 Aug 9;3(15)
JCI insight 2018 Aug 9;3(15)
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: FoxM1 Antibody [NBP1-84671] - Primary AML mononuclear cells were obtained from patients with newly diagnosed or relapsed AML. Mononuclear cells were supplemented by CD34 expansion supplement for 24 hours in the presence of drug or 0.1% DMSO. Cells were then harvested for Western blot. Treated cells were harvested and lysed using IP buffer. Protein concentration was determined. Isolated proteins were separated on SDS-PAGE and transferred to PVDF membrane. Immunoblotting was carried out with antibodies specific for FOXM1 and B-actin. This image was submitted via customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-84671] - Staining of human rectum shows moderate nuclear positivity in a subset of glandular cells.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-84671] - Staining of human skeletal muscle shows no nuclear positivity in striated muscle fibers as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-84671] - Staining of human testis shows moderate nuclear positivity in few cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: FoxM1 Antibody [NBP1-84671] - Staining of human uterine cervix shows moderate nuclear positivity in a subset of epidermal cells.
