Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-27173 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Endothelin B Receptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: Mouse brain, Rat brain.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin B Receptor using 50 µg of mouse brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin B Receptor polyclonal antibody (Product # PA5-27173) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Endothelin B Receptor using 50 µg of rat brain lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with an Endothelin B Receptor polyclonal antibody (Product # PA5-27173) at a dilution of 1:3000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173). Various tissue extracts (50 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Endothelin B Receptor Polyclonal Antibody detects Ednrb protein at membrane on U87 xenograft by immunohistochemical analysis. Sample: Paraffin-embedded U87 xenograft. Endothelin B Receptor Polyclonal Antibody (Product # PA5-27173) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.