AP8530c

antibody from Abgent

Targeting: FAS APO-1, APT1, CD95, FAS1, TNFRSF6

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

AP8530c

Provider

Abgent

Proper citation

Abgent Cat#AP8530c, RRID:AB_10552758

Product name

FAS Antibody (Center)

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

400 µl

Concentration

0.5 mg/ml

Storage

Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

References

Submitted references

The decreased expression of peroxisome proliferator-activated receptors delta (PPARdelta) is reversed by digoxin in the heart of diabetic rats.

Fan SC, Yu BC, Chen ZC, Chen LJ, Chung HH, Cheng JT
Hormone and metabolic research = Hormon- und Stoffwechselforschung = Hormones et metabolisme 2010 Aug;42(9):637-42

Western blot

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FAS Antibody (Center) (Cat. #AP8530c) in T47D cell line lysates (35ug/lane).FAS (arrow) was detected using the purified Pab.

Primary Ab dilution

1:1000

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Western blot analysis of FAS (arrow) using rabbit polyclonal FAS Antibody (Center) (Cat. #AP8530c). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the FAS gene.

Primary Ab dilution

1:1000

Immunocytochemistry

Supportive validation

Submitted by

Abgent (provider)

Main image

Experimental details

Confocal immunofluorescent analysis of FAS Antibody (Center)(Cat#AP8530c) with T47D cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).

Primary Ab dilution

1:10~50