Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunoprecipitation [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- H00000355-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000355-M01, RRID:AB_1574807
- Product name
- FAS monoclonal antibody (M01), clone 5B4
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant FAS.
- Antigen sequence
SKSVNAQVTDINSKGLELRKTVTTVETQNLEGLHH
DGQFCHKPCPPGERKARDCTVNGDEPDCVPCQEGK
EYTDKAHFSSKCRRCRLCDEGHGLEVEINC- Isotype
- IgG
- Antibody clone number
- 5B4
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of FAS expression in transfected 293T cell line by FAS monoclonal antibody (M01), clone 5B4.Lane 1: FAS transfected lysate(37.7 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged FAS is 3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of FAS transfected lysate using anti-FAS monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with FAS MaxPab rabbit polyclonal antibody.
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
