NBP1-41185
antibody from Novus Biologicals
Targeting: BRCA1
BRCC1, FANCS, PPP1R53, RNF53
Antibody data
- Antibody Data
- Antigen structure
- References [12]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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Validation data
Reference
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- Product number
- NBP1-41185 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-41185, RRID:AB_10005035
- Product name
- Mouse Monoclonal BRCA1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. This antibody does not recognize the delta exon 11 splice variant of BRCA1. In a high proportion of breast and ovarian cancer cell lines, BRCA1 aberrantly mislocates to the cytoplasm.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references BRCA immunohistochemistry for screening of BRCA mutation in epithelial ovarian cancer patients.
Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?
Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.
An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Tumor suppressor p53 is required to modulate BRCA1 expression.
Tumor suppressor p53 is required to modulate BRCA1 expression.
Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms.
ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation.
Manchana T, Tantbirojn P, Pohthipornthawat N
Gynecologic oncology reports 2020 Aug;33:100582
Gynecologic oncology reports 2020 Aug;33:100582
Can soy phytoestrogens decrease DNA methylation in BRCA1 and BRCA2 oncosuppressor genes in breast cancer?
Bosviel R, Dumollard E, Déchelotte P, Bignon YJ, Bernard-Gallon D
Omics : a journal of integrative biology 2012 May;16(5):235-44
Omics : a journal of integrative biology 2012 May;16(5):235-44
Soy phytoestrogens modify DNA methylation of GSTP1, RASSF1A, EPH2 and BRCA1 promoter in prostate cancer cells.
Vardi A, Bosviel R, Rabiau N, Adjakly M, Satih S, Dechelotte P, Boiteux JP, Fontana L, Bignon YJ, Guy L, Bernard-Gallon DJ
In vivo (Athens, Greece) 2010 Jul-Aug;24(4):393-400
In vivo (Athens, Greece) 2010 Jul-Aug;24(4):393-400
An hGCN5/TRRAP histone acetyltransferase complex co-activates BRCA1 transactivation function through histone modification.
Oishi H, Kitagawa H, Wada O, Takezawa S, Tora L, Kouzu-Fujita M, Takada I, Yano T, Yanagisawa J, Kato S
The Journal of biological chemistry 2006 Jan 6;281(1):20-6
The Journal of biological chemistry 2006 Jan 6;281(1):20-6
Differential expressions of BRCA1 and BRCA2 in infantile gynecomastia.
Bernard-Gallon DJ, Dechelotte PJ, Le Corre L, Chalabi N, Vissac-Sabatier C, Bignon YJ
Anticancer research 2004 Jan-Feb;24(1):321-4
Anticancer research 2004 Jan-Feb;24(1):321-4
BRCA1 shifts p53-mediated cellular outcomes towards irreversible growth arrest.
Ongusaha PP, Ouchi T, Kim KT, Nytko E, Kwak JC, Duda RB, Deng CX, Lee SW
Oncogene 2003 Jun 12;22(24):3749-58
Oncogene 2003 Jun 12;22(24):3749-58
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5
Breast cancer research : BCR 2001;3(1):61-5
BRCA1 and BRCA2 protein expressions in an ovotestis of a 46, XX true hermaphrodite.
Bernard-Gallon DJ, Déchelotte P, Vissac C, Aunoble B, Cravello L, Malpuech G, Bignon YJ
Breast cancer research : BCR 2001;3(1):61-5
Breast cancer research : BCR 2001;3(1):61-5
Tumor suppressor p53 is required to modulate BRCA1 expression.
Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, Aaronson SA, Lee SW
Molecular and cellular biology 2000 Oct;20(20):7450-9
Molecular and cellular biology 2000 Oct;20(20):7450-9
Tumor suppressor p53 is required to modulate BRCA1 expression.
Arizti P, Fang L, Park I, Yin Y, Solomon E, Ouchi T, Aaronson SA, Lee SW
Molecular and cellular biology 2000 Oct;20(20):7450-9
Molecular and cellular biology 2000 Oct;20(20):7450-9
Transactivation of the p21 promoter by BRCA1 splice variants in mammary epithelial cells: evidence for both common and distinct activities of wildtype and mutant forms.
Lu M, Arrick BA
Oncogene 2000 Dec 14;19(54):6351-60
Oncogene 2000 Dec 14;19(54):6351-60
ATM complexes with HDM2 and promotes its rapid phosphorylation in a p53-independent manner in normal and tumor human cells exposed to ionizing radiation.
de Toledo SM, Azzam EI, Dahlberg WK, Gooding TB, Little JB
Oncogene 2000 Dec 14;19(54):6185-93
Oncogene 2000 Dec 14;19(54):6185-93
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade detects BRCA1 protein by western blot analysis. Various whole cell extracts (30 ug) were separated by 5 % SDS-PAGE, and blotted with BRCA1 antibody [17F8] - ChIP grade BRCA1 diluted by 1:500
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: BRCA1 Antibody (17F8) [NBP1-41185] - Non-transfected (-) and transfected (+) 293T whole cell extracts (60 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with BRCA1 antibody [17F8] - ChIP grade.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - BRCA1 antibody 6B4 and BRCA1 antibody 17F8 was used for IP-WB assay. 6B4 alone (4 microgram), 17F8 alone (4 microgram), 6B4 plus 17F8 (2 microgram each), and mouse control normal IgG were used in an immunoprecipitation assay with MCF7 cell extract. Immunoprecipitated BRCA1 was detected in WB using BRCA1 antibody 6B4 at 1:1000 dilution. HeLa whole cell extract (20 microgram) was used as input in the Western blot assay.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: BRCA1 Antibody (17F8) [NBP1-41185] - ChIP grade BRCA1 antibody 6B4 and BRCA1 antibody 17F8 were used for ChIP assay. The 6B4 and 17F8 mixture (3 microgram each), or normal mouse IgG (6 microgram) were incubated with HeLa chromatin extract (100 umicrogram each) in the ChIP assay. Enrichment of genomic DNA on a BRCA1 target gene promoter (HMGA2) was validated by a Q-PCR assay.