Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- A03252 - Provider product page
- Provider
- Boster Biological Technology
- Product name
- Anti-Rac1/CDC42 Antibody
- Antibody type
- Polyclonal
- Description
- Polyclonal antibody for RAC1 detection. Host: Rabbit.Size: 100ug/vial. Tested applications: IHC. Reactive species: Human. RAC1 information: Molecular Weight: 21450 MW; Subcellular Localization: Cell membrane ; Lipid-anchor ; Cytoplasmic side . Melanosome . Cytoplasm . Inner surface of plasma membrane possibly with attachment requiring prenylation of the C-terminal cysteine (By similarity). Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts (By similarity); Tissue Specificity: Isoform B is predominantly identified in skin and epithelial tissues from the intestinal tract. Its expression is elevated in colorectal tumors at various stages of neoplastic progression, as compared to their respective adjacent tissues.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Vial size
- 100ul
- Concentration
- 0.5-1mg/ml, actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
- Storage
- At -20°C for one year. After reconstitution, at 4°C for one month. It can also be aliquoted and stored frozen at -20°C for a longer time. Avoid repeated freezing and thawing.
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Supportive validation
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- Boster Biological Technology (provider)
- Main image
- Experimental details
- Western blot analysis of lysates from NIH/3T3 cells, treated with EGF 200ng/ml 30', using Rac1/CDC42 Antibody. The lane on the right is blocked with the synthesized peptide.
- Additional image