PA5-76699

antibody from Invitrogen Antibodies

Targeting: SFTPA1 COLEC4, SFTP1, SP-A, SP-A1

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-76699

Provider

Invitrogen Antibodies

Product name

SFTPA1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Increased Alveolar Heparan Sulphate and Reduced Pulmonary Surfactant Amount and Function in the Mucopolysaccharidosis IIIA Mouse.

Paget TL, Parkinson-Lawrence EJ, Trim PJ, Autilio C, Panchal MH, Koster G, Echaide M, Snel MF, Postle AD, Morrison JL, Pérez-Gil J, Orgeig S
Cells 2021 Apr 8;10(4)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of SFTPA1. Samples were incubated with SFTPA1 polyclonal antibody (Product # PA5-76699).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 9 Protein expression of surfactant proteins -A, -B, -C and -D. ( A ) Representative Western blot images of part sets of control and MPS IIIA lung tissue samples probed for SP-A (primary antibody-rabbit Thermo Scientific PA5-76699), SP-B (primary antibody-rabbit SevenHills Bioreagents WRAB-48604), SP-C (primary antibody-rabbit SevenHills Bioreagents WRAB-MSPC) and SP-D (primary antibody-rabbit Chemicon AB3434). ( B ) Data normalised to total protein and presented as mean +- SD; two tailed Students t -tests, * indicates a significant difference, p = 0.0443 between control and MPS IIIA mice; n = 22 control, 17 MPS IIIA (SP-A analysis); 20 control, 16 MPS IIIA (SP-B analysis); 21 control, 18 MPS IIIA (SP-C analysis) and 22 control, 19 MPS IIIA (SP-D analysis) samples. Entire band height of SP-A measured from 32-45 kDa [].

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 10 Protein expression of surfactant proteins -A, -B, -C and -D in BALF. ( A ) Representative Western blot images of part sets of SP-A, -C and -D samples pictured. Western blot analyses were performed with the following primary antibodies: SP-A (rabbit Thermo Scientific PA5-76695), SP-B (rabbit SevenHills Bioreagents WRAB-48604), SP-C (rabbit SevenHills Bioreagents WRAB-MSPC) and SP-D (rabbit Thermo Scientific PA5-51476). ( B ) Data normalised to total protein and presented as mean +- SD; significant differences are indicated * p = 0.0027, ** p = 0.0024 and *** p = 0.0438; two tailed Students t -test; n = 9 control, 13 MPS IIIA (SP-A analysis); 10 control, 13 MPS IIIA (SP-B analysis); 12 control, 13 MPS IIIA (SP-C analysis) and 11 control, 13 MPS IIIA (SP-D analysis) samples. Entire band height of SP-A measured from 32-45 kDa [].