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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- NBP2-12928 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP2-12928, RRID:AB_2747514
- Product name
- Goat Polyclonal Surfactant Protein A Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. This antibody is expected to recognize all the reported isoforms of A1 (NP_005402.3
- Reactivity
- Human
- Host
- Goat
- Antigen sequence
CHLDEELQATLHDFR- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Identification of Sca-1(+)Abcg1(+) bronchioalveolar epithelial cells as the origin of lung adenocarcinoma in Gprc5a-knockout mouse model through the interaction between lung progenitor AT2 and Lgr5 cells.
Mitochondrial dysfunction in human primary alveolar type II cells in emphysema.
Epigenetic regulation of surfactant protein A gene (SP-A) expression in fetal lung reveals a critical role for Suv39h methyltransferases during development and hypoxia.
Yin H, Jiang Z, Feng X, Ji Z, Jin W
Oncogene 2020 Apr;39(18):3754-3773
Oncogene 2020 Apr;39(18):3754-3773
Mitochondrial dysfunction in human primary alveolar type II cells in emphysema.
Kosmider B, Lin CR, Karim L, Tomar D, Vlasenko L, Marchetti N, Bolla S, Madesh M, Criner GJ, Bahmed K
EBioMedicine 2019 Aug;46:305-316
EBioMedicine 2019 Aug;46:305-316
Epigenetic regulation of surfactant protein A gene (SP-A) expression in fetal lung reveals a critical role for Suv39h methyltransferases during development and hypoxia.
Benlhabib H, Mendelson CR
Molecular and cellular biology 2011 May;31(10):1949-58
Molecular and cellular biology 2011 May;31(10):1949-58
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Surfactant Protein A Antibody [NBP2-12928] - Staining of Human Lung lysate (35 ug protein in RIPA buffer). Antibody at 0.01 ug/mL. Primary incubation was 1 hour. Detected by chemiluminescence.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Surfactant Protein A Antibody [NBP2-12928] - Staining of paraffin embedded Human Lung. Antibody at 5 ug/mL. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
