Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-28795 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CDK5 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Recommended positive controls: 293T, Jurkat, Mouse brain. Predicted reactivity: Mouse (100%), Rat (100%), Pig (100%), Sheep (100%), Rhesus Monkey (100%), Bovine (100%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CDK5 using 30 µg of HeLa lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a CDK5 polyclonal antibody (Product # PA5-28795) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of CDK5 was performed by separating 30 µg of whole cell extract by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a CDK5 Polyclonal Antibody (Product # PA5-28795) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using CDK5 Polyclonal Antibody (Product # PA5-28795). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. CDK5 Polyclonal Antibody (Product # PA5-28795) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of CDK5 in paraformaldehyde-fixed A549 cells using a CDK5 polyclonal antibody (Product # PA5-28795) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- CDK5 Polyclonal Antibody detects CDK5 protein at cytosol on rat hind brain by immunohistochemical analysis. Sample: Paraffin-embedded rat hind brain. CDK5 Polyclonal Antibody (Product # PA5-28795) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded ES2 xenograft, using CDK5 (Product # PA5-28795) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.