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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [24]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- V3391 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- N-Cadherin Antibody / CDH2
- Antibody type
- Monoclonal
- Description
- Purified
- Reactivity
- Human, Mouse, Rat
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 8C11
- Vial size
- 20 µg, 100 µg (with BSA, sodium azide), 100 µg (without BSA, sodium azide), 7 ml (prediluted for IHC)
- Concentration
- 0.2 mg/ml
- Storage
- Store the CDH2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
Submitted references Hematopoietic Stem and Progenitor Cell Expansion in Contact with Mesenchymal Stromal Cells in a Hanging Drop Model Uncovers Disadvantages of 3D Culture.
Epithelial-mesenchymal transition promotes reactivity of human lung adenocarcinoma A549 cells to CpG ODN.
Exosomal Proteins as Diagnostic Biomarkers in Lung Cancer.
Loss of N-Cadherin Expression in Tumor Transplants Produced From As+3- and Cd+2-Transformed Human Urothelial (UROtsa) Cell Lines.
In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis.
Prolonged silencing of diacylglycerol acyltransferase-1 induces a dedifferentiated phenotype in human liver cells.
Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?
Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin.
Ex Vivo Maintenance of Primary Human Multiple Myeloma Cells through the Optimization of the Osteoblastic Niche.
Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.
Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma.
DDX3X induces primary EGFR-TKI resistance based on intratumor heterogeneity in lung cancer cells harboring EGFR-activating mutations.
Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
N-cadherin dependent collective cell invasion of prostate cancer cells is regulated by the N-terminus of α-catenin.
Unraveling the role of FOXQ1 in colorectal cancer metastasis.
TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages.
Transforming growth factor-β1 induces epithelial-mesenchymal transition and integrin α3β1-mediated cell migration of HSC-4 human squamous cell carcinoma cells through Slug.
Increased CD13 expression reduces reactive oxygen species, promoting survival of liver cancer stem cells via an epithelial-mesenchymal transition-like phenomenon.
High expression of GCLC is associated with malignant melanoma of low oxidative phenotype and predicts a better prognosis.
N-cadherin-mediated cell motility requires cis dimers.
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
Differential displacement of classical cadherins by VE-cadherin.
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Schmal O, Seifert J, Schäffer TE, Walter CB, Aicher WK, Klein G
Stem cells international 2016;2016:4148093
Stem cells international 2016;2016:4148093
Epithelial-mesenchymal transition promotes reactivity of human lung adenocarcinoma A549 cells to CpG ODN.
Kobayashi K, Koyama K, Suzukawa M, Igarashi S, Hebisawa A, Nagase T, Ohta K
Allergology international : official journal of the Japanese Society of Allergology 2016 Sep;65 Suppl:S45-52
Allergology international : official journal of the Japanese Society of Allergology 2016 Sep;65 Suppl:S45-52
Exosomal Proteins as Diagnostic Biomarkers in Lung Cancer.
Sandfeld-Paulsen B, Jakobsen KR, Bæk R, Folkersen BH, Rasmussen TR, Meldgaard P, Varming K, Jørgensen MM, Sorensen BS
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2016 Oct;11(10):1701-10
Journal of thoracic oncology : official publication of the International Association for the Study of Lung Cancer 2016 Oct;11(10):1701-10
Loss of N-Cadherin Expression in Tumor Transplants Produced From As+3- and Cd+2-Transformed Human Urothelial (UROtsa) Cell Lines.
Sandquist EJ, Somji S, Dunlevy JR, Garrett SH, Zhou XD, Slusser-Nore A, Sens DA
PloS one 2016 May 25;11(5):e0156310
PloS one 2016 May 25;11(5):e0156310
In patients with metastatic breast cancer the identification of circulating tumor cells in epithelial-to-mesenchymal transition is associated with a poor prognosis.
Bulfoni M, Gerratana L, Del Ben F, Marzinotto S, Sorrentino M, Turetta M, Scoles G, Toffoletto B, Isola M, Beltrami CA, Di Loreto C, Beltrami AP, Puglisi F, Cesselli D
Breast cancer research : BCR 2016 Mar 9;18(1):30
Breast cancer research : BCR 2016 Mar 9;18(1):30
Prolonged silencing of diacylglycerol acyltransferase-1 induces a dedifferentiated phenotype in human liver cells.
Chang S, Sung PS, Lee J, Park J, Shin EC, Choi C
Journal of cellular and molecular medicine 2016 Jan;20(1):38-47
Journal of cellular and molecular medicine 2016 Jan;20(1):38-47
Does smoking, age or gender affect the protein phenotype of extracellular vesicles in plasma?
Bæk R, Varming K, Jørgensen MM
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis 2016 Aug;55(1):44-52
Transfusion and apheresis science : official journal of the World Apheresis Association : official journal of the European Society for Haemapheresis 2016 Aug;55(1):44-52
Tetraspanin CD82 regulates bone marrow homing of acute myeloid leukemia by modulating the molecular organization of N-cadherin.
Marjon KD, Termini CM, Karlen KL, Saito-Reis C, Soria CE, Lidke KA, Gillette JM
Oncogene 2016 Aug 4;35(31):4132-40
Oncogene 2016 Aug 4;35(31):4132-40
Ex Vivo Maintenance of Primary Human Multiple Myeloma Cells through the Optimization of the Osteoblastic Niche.
Zhang W, Gu Y, Sun Q, Siegel DS, Tolias P, Yang Z, Lee WY, Zilberberg J
PloS one 2015 May 14;10(5):e0125995
PloS one 2015 May 14;10(5):e0125995
Terbium-based time-gated Förster resonance energy transfer imaging for evaluating protein-protein interactions on cell membranes.
Lindén S, Singh MK, Wegner KD, Regairaz M, Dautry F, Treussart F, Hildebrandt N
Dalton transactions (Cambridge, England : 2003) 2015 Mar 21;44(11):4994-5003
Dalton transactions (Cambridge, England : 2003) 2015 Mar 21;44(11):4994-5003
Exosomal proteins as potential diagnostic markers in advanced non-small cell lung carcinoma.
Jakobsen KR, Paulsen BS, Bæk R, Varming K, Sorensen BS, Jørgensen MM
Journal of extracellular vesicles 2015 Mar 2;4:26659
Journal of extracellular vesicles 2015 Mar 2;4:26659
DDX3X induces primary EGFR-TKI resistance based on intratumor heterogeneity in lung cancer cells harboring EGFR-activating mutations.
Nozaki K, Kagamu H, Shoji S, Igarashi N, Ohtsubo A, Okajima M, Miura S, Watanabe S, Yoshizawa H, Narita I
PloS one 2014 Oct 24;9(10):e111019
PloS one 2014 Oct 24;9(10):e111019
Differential in vivo tumorigenicity of distinct subpopulations from a luminal-like breast cancer xenograft.
Skrbo N, Hjortland GO, Kristian A, Holm R, Nord S, Prasmickaite L, Engebraaten O, Mælandsmo GM, Sørlie T, Andersen K
PloS one 2014 Nov 24;9(11):e113278
PloS one 2014 Nov 24;9(11):e113278
N-cadherin dependent collective cell invasion of prostate cancer cells is regulated by the N-terminus of α-catenin.
Cui Y, Yamada S
PloS one 2013;8(1):e55069
PloS one 2013;8(1):e55069
Unraveling the role of FOXQ1 in colorectal cancer metastasis.
Abba M, Patil N, Rasheed K, Nelson LD, Mudduluru G, Leupold JH, Allgayer H
Molecular cancer research : MCR 2013 Sep;11(9):1017-28
Molecular cancer research : MCR 2013 Sep;11(9):1017-28
TGF-beta1 does not induce senescence of multipotent mesenchymal stromal cells and has similar effects in early and late passages.
Walenda G, Abnaof K, Joussen S, Meurer S, Smeets H, Rath B, Hoffmann K, Fröhlich H, Zenke M, Weiskirchen R, Wagner W
PloS one 2013 Oct 17;8(10):e77656
PloS one 2013 Oct 17;8(10):e77656
Transforming growth factor-β1 induces epithelial-mesenchymal transition and integrin α3β1-mediated cell migration of HSC-4 human squamous cell carcinoma cells through Slug.
Saito D, Kyakumoto S, Chosa N, Ibi M, Takahashi N, Okubo N, Sawada S, Ishisaki A, Kamo M
Journal of biochemistry 2013 Mar;153(3):303-15
Journal of biochemistry 2013 Mar;153(3):303-15
Increased CD13 expression reduces reactive oxygen species, promoting survival of liver cancer stem cells via an epithelial-mesenchymal transition-like phenomenon.
Kim HM, Haraguchi N, Ishii H, Ohkuma M, Okano M, Mimori K, Eguchi H, Yamamoto H, Nagano H, Sekimoto M, Doki Y, Mori M
Annals of surgical oncology 2012 Jul;19 Suppl 3:S539-48
Annals of surgical oncology 2012 Jul;19 Suppl 3:S539-48
High expression of GCLC is associated with malignant melanoma of low oxidative phenotype and predicts a better prognosis.
Mougiakakos D, Okita R, Ando T, Dürr C, Gadiot J, Ichikawa J, Zeiser R, Blank C, Johansson CC, Kiessling R
Journal of molecular medicine (Berlin, Germany) 2012 Aug;90(8):935-44
Journal of molecular medicine (Berlin, Germany) 2012 Aug;90(8):935-44
N-cadherin-mediated cell motility requires cis dimers.
Kim YJ, Johnson KR, Wheelock MJ
Cell communication & adhesion 2005 Jan-Apr;12(1-2):23-39
Cell communication & adhesion 2005 Jan-Apr;12(1-2):23-39
N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane.
Wahl JK 3rd, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ
The Journal of biological chemistry 2003 May 9;278(19):17269-76
The Journal of biological chemistry 2003 May 9;278(19):17269-76
Differential displacement of classical cadherins by VE-cadherin.
Jaggi M, Wheelock MJ, Johnson KR
Cell communication & adhesion 2002 Mar-Apr;9(2):103-15
Cell communication & adhesion 2002 Mar-Apr;9(2):103-15
N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation.
Puch S, Armeanu S, Kibler C, Johnson KR, Müller CA, Wheelock MJ, Klein G
Journal of cell science 2001 Apr;114(Pt 8):1567-77
Journal of cell science 2001 Apr;114(Pt 8):1567-77
N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility.
Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR
The Journal of cell biology 2000 Dec 11;151(6):1193-206
The Journal of cell biology 2000 Dec 11;151(6):1193-206
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human brain lysate with CDH2 antibody. Predicted molecular weight ~100 kDa (unmodified), 125-140 kDa (modified).
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of FFPE mouse heart tissue with CDH2 antibody. Required HIER: boil tissue sections in 10mM Tis with pH 9 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.
