Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 368710 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#368710, RRID:AB_2571948
- Product name
- Alexa Fluor® 594 anti-human Cadherin 11
- Antibody type
- Monoclonal
- Antigen
- Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Yellow dye
- Antibody clone number
- 16G5
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- H460 Human lung Carcinoma cells were fixed with 1% paraformaldehyde (PFA), then blocked for 30 minutes with 5% FBS at room temperature. Then the cells were stained with 1.25 µg/mL anti-human Cadherin 11 (clone 16G5) Alexa Fluor® 594 (red) overnight at 4°C. Nuclei were counterstained with DRAQ7 for 15 minutes (blue). The image was captured with a 40x objective.
- Conjugate
- Yellow dye