MAB1790

antibody from R&D Systems

Targeting: CDH11 CAD11, OB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MAB1790

Provider

R&D Systems

Product name

Human Cadherin-11 Antibody

Antibody type

Monoclonal

Description

Protein A or G purified from hybridoma culture supernatant. Detects human Cadherin-11 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) Cadherin-4, rhCadherin-8, rhCadherin-12, rhCadherin-13, rhCadherin-17, rhE-Cadherin, rhN-Cadherin, rhP-Cadherin, or rhVE-Cadherin.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Antigen sequence

AAA35622


Isotype

IgG

Antibody clone number

283416

Vial size

100 ug

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

References

Submitted references

The role of cadherin-11 in microcystin-LR-induced migration and invasion in colorectal carcinoma cells.

Zhu Q, Wang Z, Zhou L, Ren Y, Gong Y, Qin W, Bai L, Hu J, Wang T
Oncology letters 2018 Feb;15(2):1417-1422

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Cadherin-11 expression is upregulated in invasive human breast cancer.

Pohlodek K, Tan YY, Singer CF, Gschwantler-Kaulich D
Oncology letters 2016 Dec;12(6):4393-4398

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Cadherin-11 in renal cell carcinoma bone metastasis.

Satcher RL, Pan T, Cheng CJ, Lee YC, Lin SC, Yu G, Li X, Hoang AG, Tamboli P, Jonasch E, Gallick GE, Lin SH
PloS one 2014;9(2):e89880

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N-cadherin-mediated cell-cell adhesion promotes cell migration in a three-dimensional matrix.

Shih W, Yamada S
Journal of cell science 2012 Aug 1;125(Pt 15):3661-70

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Hypoxia-induced abrogation of contact-dependent inhibition of rheumatoid arthritis synovial fibroblast proliferation.

Nonomura Y, Mizoguchi F, Suzuki A, Nanki T, Kato H, Miyasaka N, Kohsaka H
The Journal of rheumatology 2009 Apr;36(4):698-705

Western blot

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Cadherin-11 by Western Blot. Western blot shows lysates of PC-3 human prostate cancer cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cadherin-11 Monoclonal Antibody (Catalog # MAB1790) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Cadherin-11 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Detection of Human Cadherin-11 by Simple Western^TM. Simple Western lane view shows lysates of PC-3 human prostate cancer cell line, loaded at 0.2 mg/mL. Specific bands were detected for Cadherin-11 at approximately 114 & 137 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Cadherin-11 Monoclonal Antibody (Catalog # MAB1790). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Immunohistochemistry

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

Cadherin-11 in Human Placenta. Cadherin-11 was detected in immersion fixed paraffin-embedded sections of human placenta using Mouse Anti-Human Cadherin-11 Monoclonal Antibody (Catalog # MAB1790) at 8 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.